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A5WH99

- FADB_PSYWF

UniProt

A5WH99 - FADB_PSYWF

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Protein
Fatty acid oxidation complex subunit alpha
Gene
fadB, PsycPRwf_2100
Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate By similarity.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei120 – 1201Important for catalytic activity By similarity
Sitei140 – 1401Important for catalytic activity By similarity
Binding sitei298 – 2981Substrate By similarity
Binding sitei326 – 3261NAD; via amide nitrogen By similarity
Binding sitei345 – 3451NAD By similarity
Binding sitei409 – 4091NAD By similarity
Active sitei452 – 4521For 3-hydroxyacyl-CoA dehydrogenase activity By similarity
Binding sitei455 – 4551NAD By similarity
Binding sitei502 – 5021Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi402 – 4043NAD By similarity
Nucleotide bindingi429 – 4313NAD By similarity

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. coenzyme binding Source: InterPro
  4. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB-HAMAP
  5. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPPRW349106:GHZF-2148-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadB
Ordered Locus Names:PsycPRwf_2100
OrganismiPsychrobacter sp. (strain PRwf-1)
Taxonomic identifieri349106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000001993: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. fatty acid beta-oxidation multienzyme complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719Fatty acid oxidation complex subunit alphaUniRule annotation
PRO_1000073634Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA) By similarity.

Protein-protein interaction databases

STRINGi349106.PsycPRwf_2100.

Structurei

3D structure databases

ProteinModelPortaliA5WH99.
SMRiA5WH99. Positions 1-716.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190Enoyl-CoA hydratase/isomerase By similarity
Add
BLAST
Regioni313 – 7194073-hydroxyacyl-CoA dehydrogenase By similarity
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261344.
KOiK01825.
OMAiAKGMVMQ.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01621. FadB.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5WH99-1 [UniParc]FASTAAdd to Basket

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MIYQGNRITV SLLDDGIANM QFNAEGESVN KFDAETNKQF DEAVSALEKA    50
DNVKGLIVTS SKGVFIAGAD ITEFVSHFNK EAAEIEKWIV DINGVFNRFE 100
DLPFPKVAAI NGAALGGGCE MTLVCEYRVM GDKAQIGLPE TQLGIFPGFG 150
GSVRTPRVIG IDNAVELIAT GKAQKPAEAL KLGLVDAVVA QDDLQEAAVD 200
LVKKCIAGDL DWQAKREEKL QPVKLNQLEQ TMAFSTAKAA IFAKANPKQY 250
PAPAIAIETI EKHVNLGRDE AIKVEAAGFA KAAKTPQAES LVGLFLNDQT 300
VKKLAKQHTK NAHDINEAAV LGAGIMGGGI AYQAASKGLP IIMKDIKSEQ 350
LDLGMGEASK LLGKMVERKK MTPAQMGETL SRIRPTLNYG DFGETDIVIE 400
AVVENPKVKH AVLKEVEGLV KDNAILASNT STISITYLAT VLERPENFVG 450
MHFFNPVHRM PLVEVIRGEK SSEEAIATTV ALAQRMGKVP VVVNDCPGFL 500
VNRVLFPYFG AFDLLLKQGA DFVHVDKVME KFGWPMGPAY LIDVVGLDTG 550
VHGAEVMAEG FPDRMKPDYK GAIKHLFENN RLGQKNGVGF YKYEKDSRGK 600
PKKTADDATY ALLKDTTDTD NQQFDDQTII DRMMLAFCNE TVRCLEDNIV 650
ATPSEADMAM IMGVGFPAFR GGPCRYIDQV GLDNYLALCE KYAHLGKAYE 700
APQKIRDMAA AGETFYPKA 719
Length:719
Mass (Da):78,228
Last modified:July 10, 2007 - v1
Checksum:iA736BBCF01D446D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000713 Genomic DNA. Translation: ABQ95040.1.
RefSeqiWP_011961313.1. NC_009524.1.
YP_001280990.1. NC_009524.1.

Genome annotation databases

EnsemblBacteriaiABQ95040; ABQ95040; PsycPRwf_2100.
GeneIDi5206032.
KEGGiprw:PsycPRwf_2100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000713 Genomic DNA. Translation: ABQ95040.1 .
RefSeqi WP_011961313.1. NC_009524.1.
YP_001280990.1. NC_009524.1.

3D structure databases

ProteinModelPortali A5WH99.
SMRi A5WH99. Positions 1-716.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349106.PsycPRwf_2100.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ95040 ; ABQ95040 ; PsycPRwf_2100 .
GeneIDi 5206032.
KEGGi prw:PsycPRwf_2100.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261344.
KOi K01825.
OMAi AKGMVMQ.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci PPRW349106:GHZF-2148-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01621. FadB.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02437. FadB. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PRwf-1.

Entry informationi

Entry nameiFADB_PSYWF
AccessioniPrimary (citable) accession number: A5WH99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 10, 2007
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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