Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120Important for catalytic activityUniRule annotation1
Sitei140Important for catalytic activityUniRule annotation1
Binding sitei298SubstrateUniRule annotation1
Binding sitei326NAD; via amide nitrogenUniRule annotation1
Binding sitei345NADUniRule annotation1
Binding sitei409NADUniRule annotation1
Binding sitei431NADUniRule annotation1
Active sitei452For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei455NADUniRule annotation1
Binding sitei502SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi402 – 404NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:PsycPRwf_2100
OrganismiPsychrobacter sp. (strain PRwf-1)
Taxonomic identifieri349106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
Proteomesi
  • UP000001993 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000736341 – 719Fatty acid oxidation complex subunit alphaAdd BLAST719

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Protein-protein interaction databases

STRINGi349106.PsycPRwf_2100

Structurei

3D structure databases

ProteinModelPortaliA5WH99
SMRiA5WH99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST190
Regioni313 – 7193-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST407

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1250 LUCA
HOGENOMiHOG000261344
KOiK01825
OMAiIDLCMIL
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01621 FadB, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012799 FadB
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02437 FadB, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

A5WH99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYQGNRITV SLLDDGIANM QFNAEGESVN KFDAETNKQF DEAVSALEKA
60 70 80 90 100
DNVKGLIVTS SKGVFIAGAD ITEFVSHFNK EAAEIEKWIV DINGVFNRFE
110 120 130 140 150
DLPFPKVAAI NGAALGGGCE MTLVCEYRVM GDKAQIGLPE TQLGIFPGFG
160 170 180 190 200
GSVRTPRVIG IDNAVELIAT GKAQKPAEAL KLGLVDAVVA QDDLQEAAVD
210 220 230 240 250
LVKKCIAGDL DWQAKREEKL QPVKLNQLEQ TMAFSTAKAA IFAKANPKQY
260 270 280 290 300
PAPAIAIETI EKHVNLGRDE AIKVEAAGFA KAAKTPQAES LVGLFLNDQT
310 320 330 340 350
VKKLAKQHTK NAHDINEAAV LGAGIMGGGI AYQAASKGLP IIMKDIKSEQ
360 370 380 390 400
LDLGMGEASK LLGKMVERKK MTPAQMGETL SRIRPTLNYG DFGETDIVIE
410 420 430 440 450
AVVENPKVKH AVLKEVEGLV KDNAILASNT STISITYLAT VLERPENFVG
460 470 480 490 500
MHFFNPVHRM PLVEVIRGEK SSEEAIATTV ALAQRMGKVP VVVNDCPGFL
510 520 530 540 550
VNRVLFPYFG AFDLLLKQGA DFVHVDKVME KFGWPMGPAY LIDVVGLDTG
560 570 580 590 600
VHGAEVMAEG FPDRMKPDYK GAIKHLFENN RLGQKNGVGF YKYEKDSRGK
610 620 630 640 650
PKKTADDATY ALLKDTTDTD NQQFDDQTII DRMMLAFCNE TVRCLEDNIV
660 670 680 690 700
ATPSEADMAM IMGVGFPAFR GGPCRYIDQV GLDNYLALCE KYAHLGKAYE
710
APQKIRDMAA AGETFYPKA
Length:719
Mass (Da):78,228
Last modified:July 10, 2007 - v1
Checksum:iA736BBCF01D446D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000713 Genomic DNA Translation: ABQ95040.1
RefSeqiWP_011961313.1, NC_009524.1

Genome annotation databases

EnsemblBacteriaiABQ95040; ABQ95040; PsycPRwf_2100
KEGGiprw:PsycPRwf_2100

Similar proteinsi

Entry informationi

Entry nameiFADB_PSYWF
AccessioniPrimary (citable) accession number: A5WH99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 10, 2007
Last modified: April 25, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health