Reviewed, UniProtKB/Swiss-Prot A5WH98 (FADA_PSYWF)

Names and origin

Protein names

Recommended name:
    3-ketoacyl-CoA thiolase
    EC=2.3.1.16
Alternative name(s):
    Fatty acid oxidation complex subunit beta
    Beta-ketothiolase
    Acetyl-CoA acyltransferase

Gene names

Name:	fadA
Ordered Locus Names:	PsycPRwf_2099

Organism

Psychrobacter sp. (strain PRwf-1) [Complete proteome] [HAMAP]

Taxonomic identifier

349106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed By similarity.
Catalytic activity	Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA. HAMAP MF_01620
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01620
Subunit structure	Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: HAMAP lipid catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	3-ketoacyl-CoA thiolase HAMAP MF_01620		PRO_1000073633
Sites
	Active site	95	1	Acyl-thioester intermediate By similarity
	Active site	346	1	Proton acceptor By similarity
	Active site	376	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5WH98-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: A57A837BAA5300AF	FASTA	390	41,510
	10 20 30 40 50 60 MTTLSPKDVV IVDGVRTAMG KSKNGMFRNV RADSMSAELV RALVKRNDFD TNEVEDIIWG 70 80 90 100 110 120 CVNQTLEQGM NIGRNIGLLA DIPKTAGGQT VNRLCGSSMQ ALHTAAAQIM TNQGDVFIIG 130 140 150 160 170 180 GVEHMGHVGM MHGIDINPEA SKHYAKASNM MGLTAEMLGR MNGITREQQD EFGYESHRRA 190 200 210 220 230 240 WAATQAGRFD NEIIGIEGHD AEGRLQLCTV DEVIRPDTSM ESLAKLRPVF DPANGTVTAA 250 260 270 280 290 300 TSSALSDGAS AMLVMSAQKA KDLGLKPRAR IRSMAIAGCD AAIMGYGPVP ATQKALKRAG 310 320 330 340 350 360 LTVEDMQTIE LNEAFAAQGL SVLKALNLLD KRDIINVNGG AIALGHPLGC SGARITVTLL 370 380 390 NAMEQMDTEI GLATMCIGLG QGISTVIERV

References

[1]

"Complete sequence of chromosome of Psychrobacter sp. PRwf-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000713 Genomic DNA. Translation: ABQ95039.1.
RefSeq	YP_001280989.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5WH98.
Genome annotation databases
GeneID	5206031.
GenomeReviews	Gene locus PsycPRwf_2099 in contig CP000713_GR.
KEGG	prw:PsycPRwf_2099.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	AIDDIYW.
Family and domain databases
HAMAP	MF_01620. [Tree]
InterPro	IPR012805. FadA. IPR002155. Thiolase. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit. TIGR02445. fadA. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADA_PSYWF

Accession

Primary (citable) accession number: A5WH98

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 10, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)