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A5WGH9 (SYL_PSYWF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PsycPRwf_1830
OrganismPsychrobacter sp. (strain PRwf-1) [Complete proteome] [HAMAP]
Taxonomic identifier349106 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length885 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 885885Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334799

Regions

Motif53 – 6311"HIGH" region HAMAP-Rule MF_00049
Motif631 – 6355"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5WGH9 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 7CCF3ED1AB03D728

FASTA88599,051
        10         20         30         40         50         60 
MSTTAPEQNN PNHYHPQAIE AEQQAKWAAD KRFEVSNDIS KDANTRYMLS MFPYPSGKLH 

        70         80         90        100        110        120 
MGHVRNYTIS DVLSRYYRLK GDQVLQPMGW DAFGMPAENA AIANQVAPAK WTFSNIDNMR 

       130        140        150        160        170        180 
AQLKTLGLSI DWSREFATCT PEYYRWEQWF FLQLYKKGLV YKKLATVNWD PVDNTVLANE 

       190        200        210        220        230        240 
QVVDGKGWRS GAPVEKRDIP MYYFKITDYA DELLDDLDKL EGQWPSDVIT MQRNWIGRSQ 

       250        260        270        280        290        300 
GMEVHFPYKN SDDISTLDVF TTRPDTLMGV TYVAVAAEHR LAKLAAEKDP KIAEFCELCK 

       310        320        330        340        350        360 
KGSVAEADLA KAEKIGMDTG LTVTHPLTGE EVPVWVANYV LMSYGSGAVM AVPAHDERDF 

       370        380        390        400        410        420 
EFANKYNLPI KTVIKTPEDH EGAYTERGTL VNSGEFDGLD FDGSFEAMLA KLEPQKLAAR 

       430        440        450        460        470        480 
KIQYRLRDWG VSRQRYWGCP IPMINCEYCG NVPVDEADLP IKLPTDVVPD GRGNPLKNIP 

       490        500        510        520        530        540 
EFVNTTCPKC GGPAERETDT FDTFMESSWY YARFASPNDD TQMVEKAAAD KWLPVDQYVG 

       550        560        570        580        590        600 
GVEHAVMHLL YARFYHKLMR DEGLVSGDEP FKNLLTQGMV LAGTLYRDNP EGGKTYYFAD 

       610        620        630        640        650        660 
DVEISYDDRG QPTQAILKAD GQPVTIGKIE KMSKSKNNGV DPQTTIDQYG ADTVRLYTLF 

       670        680        690        700        710        720 
AAPADQTLEW SDDSLKGPYN FLKKVWRETQ SHLEATQELG LQVANLPAAS SIDASQLDSL 

       730        740        750        760        770        780 
AKGLRRKTHE VITKIDNDLG ERLSLNTPVS SLMELANEIG TFISKNQQIN EHTLAVQHEA 

       790        800        810        820        830        840 
LVTLLTLLSV YAPHIGEHLL EKLGVDTTRL RYPEADSAAL VQDTITMVVQ VNGKVRGKME 

       850        860        870        880 
VAPGTDAESL KAQAKAIESV AKYLTGDIKK EIVVPNKLVN IVVAG 

« Hide

References

[1]"Complete sequence of chromosome of Psychrobacter sp. PRwf-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PRwf-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000713 Genomic DNA. Translation: ABQ94770.1.
RefSeqYP_001280720.1. NC_009524.1.

3D structure databases

ProteinModelPortalA5WGH9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349106.PsycPRwf_1830.

Proteomic databases

PRIDEA5WGH9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ94770; ABQ94770; PsycPRwf_1830.
GeneID5205852.
KEGGprw:PsycPRwf_1830.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPPRW349106:GHZF-1867-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSYWF
AccessionPrimary (citable) accession number: A5WGH9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries