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A5WG08 (ASSY_PSYWF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:PsycPRwf_1659
OrganismPsychrobacter sp. (strain PRwf-1) [Complete proteome] [HAMAP]
Taxonomic identifier349106 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000070915

Regions

Nucleotide binding15 – 239ATP By similarity

Sites

Binding site421ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site931Citrulline By similarity
Binding site981Citrulline By similarity
Binding site1231ATP; via amide nitrogen By similarity
Binding site1251Aspartate By similarity
Binding site1291Aspartate By similarity
Binding site1291Citrulline By similarity
Binding site1301Aspartate By similarity
Binding site1331Citrulline By similarity
Binding site1851Citrulline By similarity
Binding site1941Citrulline By similarity
Binding site2701Citrulline By similarity
Binding site2821Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
A5WG08 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: B71C5B6CD5AF8A35

FASTA41146,272
        10         20         30         40         50         60 
MSIENKDNIN KIVLAYSGGL DTSIIAKWLQ DTYDAEVITF TADIGQGEEV EPARAKAEAM 

        70         80         90        100        110        120 
GIKHIHIEDL RAEFARDYVF PMFRANAIYE GEYLLGTSIA RPLIAKRLVE IAKEHNADAI 

       130        140        150        160        170        180 
SHGATGKGND QVRFELGAIA LAPDVKTIAP WREWDLSSRE SLMAYAKEHN IPIDFAANKK 

       190        200        210        220        230        240 
KSPYSMDANL LHISYEGDIL EDPYAEAEDD MWRWSVSPEQ APDTPEYLEL EYEKGDIVAI 

       250        260        270        280        290        300 
NGEKLEPYEV MIKLNELGGK HGIGRLDIVE NRYVGMKSRG CYETPAGTIM LKAHRGMESL 

       310        320        330        340        350        360 
TLDREAAHLK DELMPRYAKI IYNGYWFSPE RQMLQALIDK SQEYVTGTVR VKLYKGNVSV 

       370        380        390        400        410 
VGRKSPYSLF DEKIATFEDD AGAYDQKDAE GFIRLNGLRL SIEASRGRDL S 

« Hide

References

[1]"Complete sequence of chromosome of Psychrobacter sp. PRwf-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PRwf-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000713 Genomic DNA. Translation: ABQ94599.1.
RefSeqYP_001280549.1. NC_009524.1.

3D structure databases

ProteinModelPortalA5WG08.
SMRA5WG08. Positions 11-404.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349106.PsycPRwf_1659.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ94599; ABQ94599; PsycPRwf_1659.
GeneID5205349.
KEGGprw:PsycPRwf_1659.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAAPPEEAY.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycPPRW349106:GHZF-1690-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_PSYWF
AccessionPrimary (citable) accession number: A5WG08
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways