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A5WFD7

- RUVC_PSYWF

UniProt

A5WFD7 - RUVC_PSYWF

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81MagnesiumUniRule annotation
    Metal bindingi72 – 721MagnesiumUniRule annotation
    Metal bindingi144 – 1441MagnesiumUniRule annotation
    Metal bindingi147 – 1471MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPPRW349106:GHZF-1455-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:PsycPRwf_1433
    OrganismiPsychrobacter sp. (strain PRwf-1)
    Taxonomic identifieri349106 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
    ProteomesiUP000001993: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 194194Crossover junction endodeoxyribonuclease RuvCPRO_1000071034Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi349106.PsycPRwf_1433.

    Structurei

    3D structure databases

    ProteinModelPortaliA5WFD7.
    SMRiA5WFD7. Positions 2-161.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012181.
    KOiK01159.
    OMAiRQVQEMV.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5WFD7-1 [UniParc]FASTAAdd to Basket

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    MAIIIGIDPG SRMTGYGIIH QKGDTLRYLD AGTIRTDTKE MPERLKRIFQ    50
    GLTRITQYHL KYSDEPIHAA VEQVFMAENP DSALKLGQAR GAAIAALVAL 100
    DLEVSEYTAR QIKQSVCGYG AADKVQVQEM VCRILKLDVT PQQDAADGLA 150
    CAICHAHSSH SMNKLLMNST LRGRGTSKKK GRWRLTEEDL ASLK 194
    Length:194
    Mass (Da):21,384
    Last modified:July 10, 2007 - v1
    Checksum:i3CD879F64E4E1A3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000713 Genomic DNA. Translation: ABQ94378.1.
    RefSeqiWP_011960674.1. NC_009524.1.
    YP_001280328.1. NC_009524.1.

    Genome annotation databases

    EnsemblBacteriaiABQ94378; ABQ94378; PsycPRwf_1433.
    GeneIDi5206604.
    KEGGiprw:PsycPRwf_1433.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000713 Genomic DNA. Translation: ABQ94378.1 .
    RefSeqi WP_011960674.1. NC_009524.1.
    YP_001280328.1. NC_009524.1.

    3D structure databases

    ProteinModelPortali A5WFD7.
    SMRi A5WFD7. Positions 2-161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349106.PsycPRwf_1433.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ94378 ; ABQ94378 ; PsycPRwf_1433 .
    GeneIDi 5206604.
    KEGGi prw:PsycPRwf_1433.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012181.
    KOi K01159.
    OMAi RQVQEMV.
    OrthoDBi EOG6RG044.

    Enzyme and pathway databases

    BioCyci PPRW349106:GHZF-1455-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PRwf-1.

    Entry informationi

    Entry nameiRUVC_PSYWF
    AccessioniPrimary (citable) accession number: A5WFD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3