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A5WFA2 (RSMG_PSYWF) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase G

EC=2.1.1.170
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase
Gene names
Name:rsmG
Ordered Locus Names:PsycPRwf_1398
OrganismPsychrobacter sp. (strain PRwf-1) [Complete proteome] [HAMAP]
Taxonomic identifier349106 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA By similarity. HAMAP-Rule MF_00074

Catalytic activity

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA. HAMAP-Rule MF_00074

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00074.

Sequence similarities

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionrRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225Ribosomal RNA small subunit methyltransferase G HAMAP-Rule MF_00074
PRO_0000335405

Regions

Region140 – 1412S-adenosyl-L-methionine binding By similarity

Sites

Binding site891S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site941S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1571S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
A5WFA2 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 463E56121EA7034B

FASTA22525,371
        10         20         30         40         50         60 
MMNTAEFKVI FNQYDELDTL LLEAITQLKI DISAEQRRKL LLYLDKLLFW NKAYNLTAIK 

        70         80         90        100        110        120 
QPKEALIKHV IDCLAILPHL KPGKLLDIGT GAGLPGVIVA ICEPERPITV LDSNQKKIRF 

       130        140        150        160        170        180 
IRQSISELQV TNVTPVASRI ENFNPDEGDK FAVVTSRAFA SLTDFVEAAA PRLAQGGWLQ 

       190        200        210        220 
AMKGLIPEPQ ELQVLQDQWQ IDNIALSVPY LHETRHLTEL HKIVI 

« Hide

References

[1]"Complete sequence of chromosome of Psychrobacter sp. PRwf-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PRwf-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000713 Genomic DNA. Translation: ABQ94343.1.
RefSeqYP_001280293.1. NC_009524.1.

3D structure databases

ProteinModelPortalA5WFA2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349106.PsycPRwf_1398.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ94343; ABQ94343; PsycPRwf_1398.
GeneID5206695.
KEGGprw:PsycPRwf_1398.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0357.
HOGENOMHOG000221012.
KOK03501.
OMASIVPHLA.
OrthoDBEOG6HF639.

Enzyme and pathway databases

BioCycPPRW349106:GHZF-1420-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_00074. 16SrRNA_methyltr_G.
InterProIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02527. GidB. 1 hit.
[Graphical view]
PIRSFPIRSF003078. GidB. 1 hit.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00138. gidB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRSMG_PSYWF
AccessionPrimary (citable) accession number: A5WFA2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families