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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei94 – 941S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei157 – 1571S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. rRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPRW349106:GHZF-1420-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Gene namesi
Name:rsmGUniRule annotation
Ordered Locus Names:PsycPRwf_1398
OrganismiPsychrobacter sp. (strain PRwf-1)
Taxonomic identifieri349106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000001993: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Ribosomal RNA small subunit methyltransferase GPRO_0000335405Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349106.PsycPRwf_1398.

Structurei

3D structure databases

ProteinModelPortaliA5WFA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1412S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0357.
HOGENOMiHOG000221012.
KOiK03501.
OMAiIEGERCC.
OrthoDBiEOG6HF639.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

A5WFA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNTAEFKVI FNQYDELDTL LLEAITQLKI DISAEQRRKL LLYLDKLLFW
60 70 80 90 100
NKAYNLTAIK QPKEALIKHV IDCLAILPHL KPGKLLDIGT GAGLPGVIVA
110 120 130 140 150
ICEPERPITV LDSNQKKIRF IRQSISELQV TNVTPVASRI ENFNPDEGDK
160 170 180 190 200
FAVVTSRAFA SLTDFVEAAA PRLAQGGWLQ AMKGLIPEPQ ELQVLQDQWQ
210 220
IDNIALSVPY LHETRHLTEL HKIVI
Length:225
Mass (Da):25,371
Last modified:July 10, 2007 - v1
Checksum:i463E56121EA7034B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000713 Genomic DNA. Translation: ABQ94343.1.
RefSeqiWP_011960639.1. NC_009524.1.
YP_001280293.1. NC_009524.1.

Genome annotation databases

EnsemblBacteriaiABQ94343; ABQ94343; PsycPRwf_1398.
GeneIDi5206695.
KEGGiprw:PsycPRwf_1398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000713 Genomic DNA. Translation: ABQ94343.1.
RefSeqiWP_011960639.1. NC_009524.1.
YP_001280293.1. NC_009524.1.

3D structure databases

ProteinModelPortaliA5WFA2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349106.PsycPRwf_1398.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ94343; ABQ94343; PsycPRwf_1398.
GeneIDi5206695.
KEGGiprw:PsycPRwf_1398.

Phylogenomic databases

eggNOGiCOG0357.
HOGENOMiHOG000221012.
KOiK03501.
OMAiIEGERCC.
OrthoDBiEOG6HF639.

Enzyme and pathway databases

BioCyciPPRW349106:GHZF-1420-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. gidB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PRwf-1.

Entry informationi

Entry nameiRSMG_PSYWF
AccessioniPrimary (citable) accession number: A5WFA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.