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A5WFA2

- RSMG_PSYWF

UniProt

A5WFA2 - RSMG_PSYWF

Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei94 – 941S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei157 – 1571S-adenosyl-L-methionineUniRule annotation

    GO - Molecular functioni

    1. rRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPPRW349106:GHZF-1420-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
    Alternative name(s):
    16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
    Short name:
    16S rRNA m7G methyltransferaseUniRule annotation
    Gene namesi
    Name:rsmGUniRule annotation
    Ordered Locus Names:PsycPRwf_1398
    OrganismiPsychrobacter sp. (strain PRwf-1)
    Taxonomic identifieri349106 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
    ProteomesiUP000001993: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 225225Ribosomal RNA small subunit methyltransferase GPRO_0000335405Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi349106.PsycPRwf_1398.

    Structurei

    3D structure databases

    ProteinModelPortaliA5WFA2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni140 – 1412S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0357.
    HOGENOMiHOG000221012.
    KOiK03501.
    OMAiSIVPHLA.
    OrthoDBiEOG6HF639.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_00074. 16SrRNA_methyltr_G.
    InterProiIPR003682. rRNA_ssu_MeTfrase_G.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02527. GidB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003078. GidB. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00138. gidB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5WFA2-1 [UniParc]FASTAAdd to Basket

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    MMNTAEFKVI FNQYDELDTL LLEAITQLKI DISAEQRRKL LLYLDKLLFW    50
    NKAYNLTAIK QPKEALIKHV IDCLAILPHL KPGKLLDIGT GAGLPGVIVA 100
    ICEPERPITV LDSNQKKIRF IRQSISELQV TNVTPVASRI ENFNPDEGDK 150
    FAVVTSRAFA SLTDFVEAAA PRLAQGGWLQ AMKGLIPEPQ ELQVLQDQWQ 200
    IDNIALSVPY LHETRHLTEL HKIVI 225
    Length:225
    Mass (Da):25,371
    Last modified:July 10, 2007 - v1
    Checksum:i463E56121EA7034B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000713 Genomic DNA. Translation: ABQ94343.1.
    RefSeqiWP_011960639.1. NC_009524.1.
    YP_001280293.1. NC_009524.1.

    Genome annotation databases

    EnsemblBacteriaiABQ94343; ABQ94343; PsycPRwf_1398.
    GeneIDi5206695.
    KEGGiprw:PsycPRwf_1398.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000713 Genomic DNA. Translation: ABQ94343.1 .
    RefSeqi WP_011960639.1. NC_009524.1.
    YP_001280293.1. NC_009524.1.

    3D structure databases

    ProteinModelPortali A5WFA2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349106.PsycPRwf_1398.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ94343 ; ABQ94343 ; PsycPRwf_1398 .
    GeneIDi 5206695.
    KEGGi prw:PsycPRwf_1398.

    Phylogenomic databases

    eggNOGi COG0357.
    HOGENOMi HOG000221012.
    KOi K03501.
    OMAi SIVPHLA.
    OrthoDBi EOG6HF639.

    Enzyme and pathway databases

    BioCyci PPRW349106:GHZF-1420-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_00074. 16SrRNA_methyltr_G.
    InterProi IPR003682. rRNA_ssu_MeTfrase_G.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02527. GidB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003078. GidB. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00138. gidB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PRwf-1.

    Entry informationi

    Entry nameiRSMG_PSYWF
    AccessioniPrimary (citable) accession number: A5WFA2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3