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A5WDV8

- LIPA_PSYWF

UniProt

A5WDV8 - LIPA_PSYWF

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Protein

Lipoyl synthase

Gene

lipA

Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi103 – 1031Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi110 – 1101Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPRW349106:GHZF-917-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:PsycPRwf_0899
OrganismiPsychrobacter sp. (strain PRwf-1)
Taxonomic identifieri349106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000001993: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Lipoyl synthasePRO_0000325296Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349106.PsycPRwf_0899.

Structurei

3D structure databases

ProteinModelPortaliA5WDV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A5WDV8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTTVETFVP EAPPKPKKAI KGEKLRGYDK VARIPIKVIP TVEAPKKPDW
60 70 80 90 100
IRVKMSSPAE VDRIKKTLRK QKLYTVCEEA ACPNLPQCFG DGTATFMIMG
110 120 130 140 150
DICTRRCPFC DVAHGRPNEL DKDEPRHTAE TIQGLGLKYA VITSVDRDDL
160 170 180 190 200
KDGGAQHFVD VINESKALSP NCLIEILVPD FRGRMDVALD LLTETAPDVF
210 220 230 240 250
NHNIETVPRL YKAFRPGSDY QHSLDLLKQY KARRPDIATK CGFMVGLGET
260 270 280 290 300
EEEVYALLDD LKAHDVDMIT IGQYLQPSKN HAPVDRYVHP DEFQRYMDYG
310 320 330 340
KKIGFFSIWA GPMVRSSYFA DRQYYGEDCP APIRSKKALE AEGKLGC
Length:347
Mass (Da):39,119
Last modified:July 10, 2007 - v1
Checksum:iF86952D7DD78A99F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000713 Genomic DNA. Translation: ABQ93849.1.
RefSeqiWP_011960169.1. NC_009524.1.
YP_001279799.1. NC_009524.1.

Genome annotation databases

EnsemblBacteriaiABQ93849; ABQ93849; PsycPRwf_0899.
GeneIDi5204583.
KEGGiprw:PsycPRwf_0899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000713 Genomic DNA. Translation: ABQ93849.1 .
RefSeqi WP_011960169.1. NC_009524.1.
YP_001279799.1. NC_009524.1.

3D structure databases

ProteinModelPortali A5WDV8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349106.PsycPRwf_0899.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ93849 ; ABQ93849 ; PsycPRwf_0899 .
GeneIDi 5204583.
KEGGi prw:PsycPRwf_0899.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci PPRW349106:GHZF-917-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PRwf-1.

Entry informationi

Entry nameiLIPA_PSYWF
AccessioniPrimary (citable) accession number: A5WDV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 10, 2007
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3