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A5WCX0

- GLND_PSYWF

UniProt

A5WCX0 - GLND_PSYWF

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, PsycPRwf_0556
Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPPRW349106:GHZF-566-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PsycPRwf_0556
OrganismiPsychrobacter sp. (strain PRwf-1)
Taxonomic identifieri349106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000001993: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 899899Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000071929Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349106.PsycPRwf_0556.

Structurei

3D structure databases

ProteinModelPortaliA5WCX0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini494 – 57784HD
Add
BLAST
Domaini719 – 80486ACT 1
Add
BLAST
Domaini827 – 89973ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 347347UridylyltransferaseUniRule annotation
Add
BLAST
Regioni348 – 718371Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5WCX0-1 [UniParc]FASTAAdd to Basket

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MFISDPTDSL ITPLPDLTAH SEAQSRNLGI PDWLKQIEAD IATALEKGVD    50
IRHLVEARAA SIDSLLIELF KLHELTQTDL ALFAVGGYGR GELSPYSDVD 100
ILILSPDTLS AEISQKVDGF VARLWDVGIE PGIAVRTIED CLQVATDITV 150
ATNLLEARLL IGNDSLSAIP NNVVQQTWSQ KEFYDAKMAE ARARHLLHNG 200
TEYNLEPDIK KSPGGLRDIH TIGWITKRYF RITKLYDLVP QDFLTEKEFD 250
ELMFAEGFLW RIRHHLHHLT GRNENKLLFD YQRDIAERMG YEQSEEDEPN 300
AAVENFMRDY YRCAMQISTL SEMLTSHYYE TLIEARLPES ERPEKSVLNA 350
RFNRVGDHIA IAHHHVFAQH PEAILEMFLL MGQHGIKHIR TRTLRALKIA 400
ARGIDQHYRD NPLHKKLFLD NLKEQNYLFH RLRIMKRYGV LANYMPQFVN 450
LIGLMQYDLF HRYTVDAHIL LLIRMLHRFT SPKYQDDFAL VGSIYKRIER 500
KEIIVLAAIF HDIAKGRGGD HSELGERDAI EFCLSHGMSE ADAKLIGWLT 550
RYHLLMSMTA QKQDISDPEV VTKFANLVGN VTHLNHLYVL TVADMNATNP 600
QLWNSWRASL MKQLYTQTRR ILRADLDAPT NRQEMIATTR QQALTMLDKV 650
DNQHMNREEV LALWDELGDE YFLREIPEAI MWHTEAILNH PPIGRASDAN 700
SEPLIVLREH RELALDAVQI FIYTQDQANL FAVTMAVFDQ MNLDVLDARI 750
ITATRDFALD SYVLLDRHGT LLTDPESREE LTRRLIDAFK NPETPKLVQK 800
RLPRRLKNFQ VPTTIDFNYN EASRQHVMSL TTLDQPGLLA RIGQVFLNEG 850
IEVHAARITT LGERAEDMFY ISDIGDNMLS DAKLERLRTT LIDVLTPSC 899
Length:899
Mass (Da):103,339
Last modified:July 10, 2007 - v1
Checksum:i52396B5DE7317311
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000713 Genomic DNA. Translation: ABQ93511.1.
RefSeqiWP_011959838.1. NC_009524.1.
YP_001279461.1. NC_009524.1.

Genome annotation databases

EnsemblBacteriaiABQ93511; ABQ93511; PsycPRwf_0556.
GeneIDi5204669.
KEGGiprw:PsycPRwf_0556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000713 Genomic DNA. Translation: ABQ93511.1 .
RefSeqi WP_011959838.1. NC_009524.1.
YP_001279461.1. NC_009524.1.

3D structure databases

ProteinModelPortali A5WCX0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349106.PsycPRwf_0556.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ93511 ; ABQ93511 ; PsycPRwf_0556 .
GeneIDi 5204669.
KEGGi prw:PsycPRwf_0556.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PPRW349106:GHZF-566-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PRwf-1.

Entry informationi

Entry nameiGLND_PSYWF
AccessioniPrimary (citable) accession number: A5WCX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 10, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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