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A5WCU0 (DDL_PSYWF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:PsycPRwf_0526
OrganismPsychrobacter sp. (strain PRwf-1) [Complete proteome] [HAMAP]
Taxonomic identifier349106 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341157

Regions

Domain132 – 329198ATP-grasp
Nucleotide binding158 – 21356ATP By similarity

Sites

Metal binding2831Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 2 By similarity
Metal binding2981Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5WCU0 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 4A2A9B0B46BCB587

FASTA33235,848
        10         20         30         40         50         60 
MPMTMTQSAT NPTATPVSAN KASANAATTQ DPSIFGKVAV VFGGNSNERA VSLDSGNAVL 

        70         80         90        100        110        120 
QALQSQGIDA THFDPKDQDV TELKNYDRVF NVLHGRGGED GQLQGLLDWL GLPQTGSGVL 

       130        140        150        160        170        180 
ASAIGMDKVR TKQLWHGCGL STAPFAVLGA DTDWQQIVNT LGLPLIVKPV HEGSSIGMSK 

       190        200        210        220        230        240 
VNTLDELPKA YEVAAGCGDV VMAEKWITGR EFTIVIIDDE AYPVIRLQPA DISNFYDYEA 

       250        260        270        280        290        300 
KYNRNDTQYH IPCGLSAEEE AHLQALSLAA FKAVDAKGWG RIDAMQDEEG NFWLLEINTV 

       310        320        330 
PGMTSHSLVP MAAKARGMNF EQLCWHILAQ TL 

« Hide

References

[1]"Complete sequence of chromosome of Psychrobacter sp. PRwf-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PRwf-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000713 Genomic DNA. Translation: ABQ93481.1.
RefSeqYP_001279431.1. NC_009524.1.

3D structure databases

ProteinModelPortalA5WCU0.
SMRA5WCU0. Positions 36-329.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349106.PsycPRwf_0526.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ93481; ABQ93481; PsycPRwf_0526.
GeneID5205497.
KEGGprw:PsycPRwf_0526.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycPPRW349106:GHZF-536-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PSYWF
AccessionPrimary (citable) accession number: A5WCU0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 10, 2007
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways