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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi491 – 4911MagnesiumUniRule annotation
Metal bindingi497 – 4971MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPPRW349106:GHZF-168-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:PsycPRwf_0164
OrganismiPsychrobacter sp. (strain PRwf-1)
Taxonomic identifieri349106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
ProteomesiUP000001993 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 704704Polyribonucleotide nucleotidyltransferasePRO_0000329792Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi349106.PsycPRwf_0164.

Structurei

3D structure databases

ProteinModelPortaliA5WBT2.
SMRiA5WBT2. Positions 622-695.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini558 – 61760KHUniRule annotationAdd
BLAST
Domaini627 – 69569S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5WBT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMFNTISRE FQYGNEQVVI ETGRIARQAN SVLVHMGGVS VLVAVVVKSD
60 70 80 90 100
AKEGQNFFPL TVNYQEKMYA AGKIPGAYGK REGRPTEFET LTSRLIDRPI
110 120 130 140 150
RPLFPEGYVN EIQITATVIS SDKKHYADIA AMIGASAALS ISDAPFNGPI
160 170 180 190 200
GGARVGFING EYVLNPSIEE LKSSDLDLVV AGTKSAVLMV ESEAAELSED
210 220 230 240 250
QMLGAVLYGH EQQQIVIDNI AELAKAVGTQ KQDFVAPELN AELKEKVTTQ
260 270 280 290 300
FGEQVAEAYA ISDKQARYEK LDEIKAAAID ALAGDPESEE FAEKEAQIKE
310 320 330 340 350
IYNDLKYRTV RDAILSGKPR IDGRDLDTVR ALDIQVGVLP YTHGSALFTR
360 370 380 390 400
GETQALVTTT LGNSRDVNLI DSLAGTIQDH FMLHYNFPHY SVGETGREGV
410 420 430 440 450
PKRREIGHGR LARRGVQAML PDSDRFPYVI RVVSEITESN GSSSMASVCG
460 470 480 490 500
ASLALMDAGV PLKAPVAGIA MGLVKEGDRF AVLSDILGDE DHLGDMDFKV
510 520 530 540 550
AGTKDGITAL QMDIKIEGIT SDIMEKALEQ AHAGRIHILN AMNEVLPASR
560 570 580 590 600
TEINAHAPNY AVIEINSDKI RDVIGKGGAT IRQLTEDTGA VIDIDDNGTI
610 620 630 640 650
RIFGENKAAT KEAIRQIEAI TAEVEVGKVY KGTVARVVDF GAFVTVLPGT
660 670 680 690 700
DGLVHISQIA DERVESVSDY LSEGQQINVL VQDVDNRGRI KLTMKGVEQE

QTQA
Length:704
Mass (Da):76,265
Last modified:July 10, 2007 - v1
Checksum:i4D28EEC2F314B94B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000713 Genomic DNA. Translation: ABQ93123.1.
RefSeqiWP_011959459.1. NC_009524.1.
YP_001279073.1. NC_009524.1.

Genome annotation databases

EnsemblBacteriaiABQ93123; ABQ93123; PsycPRwf_0164.
KEGGiprw:PsycPRwf_0164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000713 Genomic DNA. Translation: ABQ93123.1.
RefSeqiWP_011959459.1. NC_009524.1.
YP_001279073.1. NC_009524.1.

3D structure databases

ProteinModelPortaliA5WBT2.
SMRiA5WBT2. Positions 622-695.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349106.PsycPRwf_0164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ93123; ABQ93123; PsycPRwf_0164.
KEGGiprw:PsycPRwf_0164.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciPPRW349106:GHZF-168-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PRwf-1.

Entry informationi

Entry nameiPNP_PSYWF
AccessioniPrimary (citable) accession number: A5WBT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 10, 2007
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.