A5WBQ0 (SYE_PSYWF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	PsycPRwf_0131

Organism

Psychrobacter sp. (strain PRwf-1) [Complete proteome] [HAMAP]

Taxonomic identifier

349106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000330991
Regions
	Motif	16 – 26	11	"HIGH" region HAMAP MF_00022_B
	Motif	257 – 261	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	260	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5WBQ0 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 28326DE12067A3D8	FASTA	505	57,225
	10 20 30 40 50 60 MTQTTAQRPV RTRIAPSPTG FPHVGTAYIA LFNLAFAKAH GGEFILRIED TDQSRSTEQS 70 80 90 100 110 120 EQMILDALRW VGLDWAEGPD NGGPHAPYRQ SERSEIYKQY AQELLDKGHA FRCFCTPEEL 130 140 150 160 170 180 DAMRAEQIAR GENPHYDGRY ANLPREESDK MAAEGKPFVI RMKVPTEGVC KVQDMLRGEV 190 200 210 220 230 240 EIPWEQVDMQ VLLKTDGLPT YHLANVVDDH LMEITHVIRG EEWLNSAPKH QLLYDYFGWE 250 260 270 280 290 300 MPVLCHMPLL RNPDKSKLSK RKNPTSITYY RDAGVLPEAL LNYLGRMGYS MPDDKEIFTL 310 320 330 340 350 360 DEMIESFDIT RVSLGGPIFD LEKLHWLNGE WLRALTPEQL KNKILDWAND SDKLTAIAAA 370 380 390 400 410 420 IQPRIHLLSD AVNWGGFYFQ NLPSITAESF EHKTLTPEQI MEILQIATWK LEALPEWSED 430 440 450 460 470 480 NIFKTLKGLS AALDIKLRDM MAPFFIAIAG STSSTPVMNS MYVIGADMTL TRLRHAVEVL 490 500 GGIGKKKLKK LEKTAADMPD FLAAE

References

[1]

"Complete sequence of chromosome of Psychrobacter sp. PRwf-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PRwf-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000713 Genomic DNA. Translation: ABQ93091.1.
RefSeq	YP_001279041.1. NC_009524.1.
3D structure databases
ProteinModelPortal	A5WBQ0.
ModBase	Search...
Protein-protein interaction databases
STRING	A5WBQ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5204558.
GenomeReviews	Gene locus PsycPRwf_0131 in contig CP000713_GR.
KEGG	prw:PsycPRwf_0131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DKETAND.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PSP56811:PSYCPRWF_0131-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PSYWF

Accession

Primary (citable) accession number: A5WBQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program