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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei88 – 881S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPUT351746:GI26-5397-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Gene namesi
Name:rsmGUniRule annotation
Ordered Locus Names:Pput_5305
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006553 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Ribosomal RNA small subunit methyltransferase GPRO_1000010187Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_5305.

Structurei

3D structure databases

ProteinModelPortaliA5WBB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1352S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
KOiK03501.
OMAiMVKHILD.
OrthoDBiEOG6HF639.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

A5WBB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLVTPQHA EELSTGARQL GVELTAEQHE KLLGYLALLI KWNKAYNLTA
60 70 80 90 100
VRDPDEMVSR HLLDSLSVMS FIHNDRDNWL DVGSGGGMPG IPLAILHPHK
110 120 130 140 150
RVTVLDANGK KTRFLTQVKM ELKLDNLTVI HSRVEAFQPA QPFDGIISRA
160 170 180 190 200
FSSMENFTNW TRHLGDTGTQ WLAMKGLHPA DELVALPADF TVESEQALTV
210
PGCQGQRHLL ILRRKA
Length:216
Mass (Da):24,064
Last modified:July 10, 2007 - v1
Checksum:i1C20B3FAA5E6AA1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ81423.1.
RefSeqiWP_003253179.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ81423; ABQ81423; Pput_5305.
KEGGippf:Pput_5305.
PATRICi19926880. VBIPsePut56420_5381.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ81423.1.
RefSeqiWP_003253179.1. NC_009512.1.

3D structure databases

ProteinModelPortaliA5WBB3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_5305.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ81423; ABQ81423; Pput_5305.
KEGGippf:Pput_5305.
PATRICi19926880. VBIPsePut56420_5381.

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
KOiK03501.
OMAiMVKHILD.
OrthoDBiEOG6HF639.

Enzyme and pathway databases

BioCyciPPUT351746:GI26-5397-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiRSMG_PSEP1
AccessioniPrimary (citable) accession number: A5WBB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: November 11, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.