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A5WB10 (RNPH_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease PH

Short name=RNase PH
EC=2.7.7.56
Alternative name(s):
tRNA nucleotidyltransferase
Gene names
Name:rph
Ordered Locus Names:Pput_5202
OrganismPseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]
Taxonomic identifier351746 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564

Catalytic activity

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564

Sequence similarities

Belongs to the RNase PH family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiontRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA nucleotidyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

tRNA-specific ribonuclease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Ribonuclease PH HAMAP-Rule MF_00564
PRO_1000024852

Sequences

Sequence LengthMass (Da)Tools
A5WB10 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: E94D741962E77142

FASTA24025,723
        10         20         30         40         50         60 
MKRPSGRVAD QLRSIRITRN YTKHAEGSVL VEFGDTKVIC TVSVENGVPR FLKGQGQGWL 

        70         80         90        100        110        120 
TAEYGMLPRS TGERNQREAS RGKQGGRTLE IQRLIGRSLR AALDMSKLGD ITLYVDCDVI 

       130        140        150        160        170        180 
QADGGTRTAS ITGAMVALCD ALAVIKKRGG LKGGNPLKHM IAAVSVGMYQ GEAVLDLDYL 

       190        200        210        220        230        240 
EDSAAETDLN VVMTSAGGFI EVQGTAEGAP FQPEDFNAML ALAQKGMNEI FELQQAALAD 

« Hide

References

[1]"Complete sequence of Pseudomonas putida F1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F1 / ATCC 700007.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000712 Genomic DNA. Translation: ABQ81320.1.
RefSeqYP_001270504.1. NC_009512.1.

3D structure databases

ProteinModelPortalA5WB10.
SMRA5WB10. Positions 1-240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351746.Pput_5202.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ81320; ABQ81320; Pput_5202.
GeneID5192830.
KEGGppf:Pput_5202.
PATRIC19926672. VBIPsePut56420_5277.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0689.
HOGENOMHOG000229516.
KOK00989.
OMAERTIQID.
OrthoDBEOG6CZQQP.

Enzyme and pathway databases

BioCycPPUT351746:GI26-5295-MONOMER.

Family and domain databases

Gene3D3.30.230.70. 1 hit.
HAMAPMF_00564. RNase_PH.
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsTIGR01966. RNasePH. 1 hit.
PROSITEPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNPH_PSEP1
AccessionPrimary (citable) accession number: A5WB10
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families