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Protein

Ribonuclease PH

Gene

rph

Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

GO - Molecular functioni

  1. tRNA binding Source: UniProtKB-HAMAP
  2. tRNA nucleotidyltransferase activity Source: UniProtKB-HAMAP
  3. tRNA-specific ribonuclease activity Source: InterPro

GO - Biological processi

  1. tRNA processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciPPUT351746:GI26-5295-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Ordered Locus Names:Pput_5202
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006553 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Ribonuclease PHPRO_1000024852Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_5202.

Structurei

3D structure databases

ProteinModelPortaliA5WB10.
SMRiA5WB10. Positions 1-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0689.
HOGENOMiHOG000229516.
KOiK00989.
OMAiGKGQGWV.
OrthoDBiEOG6CZQQP.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5WB10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRPSGRVAD QLRSIRITRN YTKHAEGSVL VEFGDTKVIC TVSVENGVPR
60 70 80 90 100
FLKGQGQGWL TAEYGMLPRS TGERNQREAS RGKQGGRTLE IQRLIGRSLR
110 120 130 140 150
AALDMSKLGD ITLYVDCDVI QADGGTRTAS ITGAMVALCD ALAVIKKRGG
160 170 180 190 200
LKGGNPLKHM IAAVSVGMYQ GEAVLDLDYL EDSAAETDLN VVMTSAGGFI
210 220 230 240
EVQGTAEGAP FQPEDFNAML ALAQKGMNEI FELQQAALAD
Length:240
Mass (Da):25,723
Last modified:July 10, 2007 - v1
Checksum:iE94D741962E77142
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ81320.1.
RefSeqiYP_001270504.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ81320; ABQ81320; Pput_5202.
KEGGippf:Pput_5202.
PATRICi19926672. VBIPsePut56420_5277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ81320.1.
RefSeqiYP_001270504.1. NC_009512.1.

3D structure databases

ProteinModelPortaliA5WB10.
SMRiA5WB10. Positions 1-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_5202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ81320; ABQ81320; Pput_5202.
KEGGippf:Pput_5202.
PATRICi19926672. VBIPsePut56420_5277.

Phylogenomic databases

eggNOGiCOG0689.
HOGENOMiHOG000229516.
KOiK00989.
OMAiGKGQGWV.
OrthoDBiEOG6CZQQP.

Enzyme and pathway databases

BioCyciPPUT351746:GI26-5295-MONOMER.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiRNPH_PSEP1
AccessioniPrimary (citable) accession number: A5WB10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: April 1, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.