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A5WB10

- RNPH_PSEP1

UniProt

A5WB10 - RNPH_PSEP1

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Protein
Ribonuclease PH
Gene
rph, Pput_5202
Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

GO - Molecular functioni

  1. tRNA binding Source: InterPro
  2. tRNA nucleotidyltransferase activity Source: UniProtKB-EC
  3. tRNA-specific ribonuclease activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciPPUT351746:GI26-5295-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PH (EC:2.7.7.56)
Short name:
RNase PH
Alternative name(s):
tRNA nucleotidyltransferase
Gene namesi
Name:rph
Ordered Locus Names:Pput_5202
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006553: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Ribonuclease PHUniRule annotation
PRO_1000024852Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_5202.

Structurei

3D structure databases

ProteinModelPortaliA5WB10.
SMRiA5WB10. Positions 1-240.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.

Phylogenomic databases

eggNOGiCOG0689.
HOGENOMiHOG000229516.
KOiK00989.
OMAiERTIQID.
OrthoDBiEOG6CZQQP.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5WB10-1 [UniParc]FASTAAdd to Basket

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MKRPSGRVAD QLRSIRITRN YTKHAEGSVL VEFGDTKVIC TVSVENGVPR    50
FLKGQGQGWL TAEYGMLPRS TGERNQREAS RGKQGGRTLE IQRLIGRSLR 100
AALDMSKLGD ITLYVDCDVI QADGGTRTAS ITGAMVALCD ALAVIKKRGG 150
LKGGNPLKHM IAAVSVGMYQ GEAVLDLDYL EDSAAETDLN VVMTSAGGFI 200
EVQGTAEGAP FQPEDFNAML ALAQKGMNEI FELQQAALAD 240
Length:240
Mass (Da):25,723
Last modified:July 10, 2007 - v1
Checksum:iE94D741962E77142
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000712 Genomic DNA. Translation: ABQ81320.1.
RefSeqiYP_001270504.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ81320; ABQ81320; Pput_5202.
GeneIDi5192830.
KEGGippf:Pput_5202.
PATRICi19926672. VBIPsePut56420_5277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000712 Genomic DNA. Translation: ABQ81320.1 .
RefSeqi YP_001270504.1. NC_009512.1.

3D structure databases

ProteinModelPortali A5WB10.
SMRi A5WB10. Positions 1-240.
ModBasei Search...

Protein-protein interaction databases

STRINGi 351746.Pput_5202.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ81320 ; ABQ81320 ; Pput_5202 .
GeneIDi 5192830.
KEGGi ppf:Pput_5202.
PATRICi 19926672. VBIPsePut56420_5277.

Phylogenomic databases

eggNOGi COG0689.
HOGENOMi HOG000229516.
KOi K00989.
OMAi ERTIQID.
OrthoDBi EOG6CZQQP.

Enzyme and pathway databases

BioCyci PPUT351746:GI26-5295-MONOMER.

Family and domain databases

Gene3Di 3.30.230.70. 1 hit.
HAMAPi MF_00564. RNase_PH.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view ]
Pfami PF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsi TIGR01966. RNasePH. 1 hit.
PROSITEi PS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiRNPH_PSEP1
AccessioniPrimary (citable) accession number: A5WB10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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