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Protein

Thymidylate synthase

Gene

thyA

Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei159 – 1591UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciPPUT351746:GI26-5136-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Pput_5043
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006553 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277Thymidylate synthasePRO_1000000654Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351746.Pput_5043.

Structurei

3D structure databases

ProteinModelPortaliA5WAK1.
SMRiA5WAK1. Positions 2-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
KOiK00560.
OMAiKVVDYKH.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.

Sequencei

Sequence statusi: Complete.

A5WAK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSYLDLLTD VLENGSVKGD RTGTGTISVF GRQYRHDLSK GFPLLTTKKL
60 70 80 90 100
HFKSIVNELI WFLSGDTNIK WLNENGVKIW NEWATEEGDL GPVYGKQWTA
110 120 130 140 150
WPTKDGGTIN QIDYVVNALK TNPNSRRILF HGWNVEYLPD EKVSPQENAE
160 170 180 190 200
NGKMALPPCH LLYQFYVVDN KLSAHLFIRS SDSFLGLPYN TASLACLTHM
210 220 230 240 250
LAQQCDMGVG EIVISLSDVH IYSNHMEQVK TQISREPRKL PELKLLRKPE
260 270
SIYDYKFEDF ELVGYDPHPH IAAPVSI
Length:277
Mass (Da):31,541
Last modified:July 10, 2007 - v1
Checksum:i1EF254D48F73EA77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ81161.1.
RefSeqiWP_012054009.1. NC_009512.1.
YP_001270345.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ81161; ABQ81161; Pput_5043.
KEGGippf:Pput_5043.
PATRICi19926354. VBIPsePut56420_5118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ81161.1.
RefSeqiWP_012054009.1. NC_009512.1.
YP_001270345.1. NC_009512.1.

3D structure databases

ProteinModelPortaliA5WAK1.
SMRiA5WAK1. Positions 2-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_5043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ81161; ABQ81161; Pput_5043.
KEGGippf:Pput_5043.
PATRICi19926354. VBIPsePut56420_5118.

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
KOiK00560.
OMAiKVVDYKH.
OrthoDBiEOG6K6V53.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciPPUT351746:GI26-5136-MONOMER.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiTYSY_PSEP1
AccessioniPrimary (citable) accession number: A5WAK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: April 29, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.