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A5WAD6

- TRMB_PSEP1

UniProt

A5WAD6 - TRMB_PSEP1

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, Pput_4976
Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701S-adenosyl-L-methionine By similarity
Binding sitei95 – 951S-adenosyl-L-methionine By similarity
Binding sitei122 – 1221S-adenosyl-L-methionine By similarity
Active sitei145 – 1451 By similarity
Binding sitei145 – 1451S-adenosyl-L-methionine By similarity
Binding sitei149 – 1491Substrate By similarity
Binding sitei181 – 1811Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPPUT351746:GI26-5069-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:Pput_4976
    OrganismiPseudomonas putida (strain F1 / ATCC 700007)
    Taxonomic identifieri351746 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000006553: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_1000064405Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi351746.Pput_4976.

    Structurei

    3D structure databases

    ProteinModelPortaliA5WAD6.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2214Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiTSATNDY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5WAD6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTESHDTPIT SDGEARPHRR IKSFVMRAGR MTEGQQRGLD QGGPLYILPL    50
    ADSPVDYDQV FGRSAPRTLE IGFGMGHSLL EMAAAAPEQD FIGVEVHRPG 100
    VGALLNGVLT QGLKNLRVYD CDAIEVLNRC VADNSLDRLM LFFPDPWHKA 150
    RHHKRRIVQL EFAELVRRKL KPGGVFHMAT DWEPYAEYML EVMSAAPGYR 200
    NRAADGTYVP RPEERPITKF ERRGERLGHG VWDLKFEKVD 240
    Length:240
    Mass (Da):27,193
    Last modified:July 10, 2007 - v1
    Checksum:i55BE8298D8E1E6C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000712 Genomic DNA. Translation: ABQ81096.1.
    RefSeqiYP_001270280.1. NC_009512.1.

    Genome annotation databases

    EnsemblBacteriaiABQ81096; ABQ81096; Pput_4976.
    GeneIDi5193705.
    KEGGippf:Pput_4976.
    PATRICi19926218. VBIPsePut56420_5049.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000712 Genomic DNA. Translation: ABQ81096.1 .
    RefSeqi YP_001270280.1. NC_009512.1.

    3D structure databases

    ProteinModelPortali A5WAD6.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 351746.Pput_4976.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ81096 ; ABQ81096 ; Pput_4976 .
    GeneIDi 5193705.
    KEGGi ppf:Pput_4976.
    PATRICi 19926218. VBIPsePut56420_5049.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi TSATNDY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci PPUT351746:GI26-5069-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: F1 / ATCC 700007.

    Entry informationi

    Entry nameiTRMB_PSEP1
    AccessioniPrimary (citable) accession number: A5WAD6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: July 10, 2007
    Last modified: June 11, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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