Reviewed, UniProtKB/Swiss-Prot A5WA97 (BETA_PSEP1)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Pput_4937

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Choline dehydrogenase HAMAP MF_00750		PRO_1000046570
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5WA97-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: F06375A1B1FE06A8	FASTA	565	62,450
	10 20 30 40 50 60 MSQEFDYIIV GAGSAGNTLA TRLTEDAGVT VLLLEAGGPD YRFDFRTQMP AALAFPLQGR 70 80 90 100 110 120 RYNWAYETDP EPYMDGRRME CGRGKGLGGS SLINGMCYIR GNAMDFDGWA ELPGLEDWTY 130 140 150 160 170 180 LDCLPYFRKA ETRDIGPNDY HGGDGPVSVA TPKAGNNPLF HAMVEAGVQA GYPRTEDLNG 190 200 210 220 230 240 YQQEGFGPMD RSVTKNGRRS STARGYLDQA KKRPNLTIVT HALTDRVLFD GKRAVGVTYL 250 260 270 280 290 300 VGDSEERVEA RARKEVIVSS GAIASPQLLQ RSGVGPRALL ESLDIPVVHD LPGVGENLQD 310 320 330 340 350 360 HLELYLQYAC TQPVSLYPSL LWWNQPAIGA EWLFNGTGIG ASNQFEAGGF IRTRPEFKWP 370 380 390 400 410 420 NIQYHFLPVA INYNGSNGVK EHGFQAHMGS MRSPARGRIQ AKSKDPRQHP SILFNYMSTE 430 440 450 460 470 480 QDWQEFRDGI RLTREIMAQP ALDPYRGREI SPGAHVQTDE ELDKFIREHA ETAFHPSCSC 490 500 510 520 530 540 KMGTDDMAVV DGEGRVHGMK GLRVVDASIM PLIITGNLNA TTIMIAEKIS DKIRGRKPLP 550 560 RSTAKYYVAG DAPVKGKPMR EVKQG

References

[1]

"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000712 Genomic DNA. Translation: ABQ81057.1.
RefSeq	YP_001270241.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5194007.
GenomeReviews	Gene locus Pput_4937 in contig CP000712_GR.
KEGG	ppf:Pput_4937.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A5WA97. GRRMECG.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_PSEP1

Accession

Primary (citable) accession number: A5WA97

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)