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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurH
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
Alternative name(s):
AICAR transformylase
IMP cyclohydrolase (EC:3.5.4.10)
Alternative name(s):
ATIC
IMP synthase
Inosinicase
Gene namesi
Name:purH
Ordered Locus Names:Pput_4697
OrganismiPseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006553 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000189411 – 535Bifunctional purine biosynthesis protein PurHAdd BLAST535

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_4697.

Structurei

3D structure databases

ProteinModelPortaliA5W9K7.
SMRiA5W9K7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.

Sequence similaritiesi

Belongs to the PurH family.

Phylogenomic databases

eggNOGiENOG4105DC1. Bacteria.
COG0138. LUCA.
HOGENOMiHOG000230373.
KOiK00602.
OMAiDLLFAWK.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH. 1 hit.
InterProiView protein in InterPro
IPR024051. AICAR_Tfase_dup_dom_sf.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
IPR036914. MGS-like_dom_sf.
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiView protein in Pfam
PF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiView protein in SMART
SM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

A5W9K7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQTTRLPV RRALISVSDK TGILEFAREL QQLGVEILST GGTYKLLKDN
60 70 80 90 100
GVNAVEVADY TGFAEMMDGR VKTLHPKIHG GILGRRGTDD AIMNEHGIKP
110 120 130 140 150
IDLVAVNLYP FEATISKPGC DLPTAIENID IGGPTMVRSA AKNHKDVAIV
160 170 180 190 200
VNASDYAGIV EGLKAGGLTY AQRFDLMLKA FEHTAAYDGM IANYMGTIDQ
210 220 230 240 250
AKDTLSTEAR SEFPRTFNSQ FVKAQEMRYG ENPHQSAAFY VEAKKGEASI
260 270 280 290 300
STAVQLQGKE LSFNNVADTD AALECVKSFV KPACVIVKHA NPCGVAVVPE
310 320 330 340 350
DEGGIRKAYD LAYATDTESA FGGIIAFNRE LDGETAKAIV DRQFVEVIIA
360 370 380 390 400
PKISQAARDV VAAKQNVRLL ECGEWPAERA AGWDFKRVNG GLLVQSRDIG
410 420 430 440 450
MITADDLKIV TKRAPTEQEI HDLVFAWKVA KFVKSNAIVY AKQRQTIGVG
460 470 480 490 500
AGQMSRVNSA RIAAIKAEHA GLQVQGAVMA SDAFFPFRDG IDNAAKVGIS
510 520 530
AVIQPGGSMR DAEVIAAADE AGIAMVFTGM RHFRH
Length:535
Mass (Da):57,696
Last modified:July 10, 2007 - v1
Checksum:iAB1217C6FD569DDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ80817.1.
RefSeqiWP_003249687.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ80817; ABQ80817; Pput_4697.
KEGGippf:Pput_4697.

Similar proteinsi

Entry informationi

Entry nameiPUR9_PSEP1
AccessioniPrimary (citable) accession number: A5W9K7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: November 22, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families