SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A5W9I5

- LIPA_PSEP1

UniProt

A5W9I5 - LIPA_PSEP1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Lipoyl synthase
Gene
lipA, Pput_4675
Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi117 – 1171Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPUT351746:GI26-4766-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Pput_4675
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006553: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Lipoyl synthaseUniRule annotation
PRO_1000012258Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_4675.

Structurei

3D structure databases

ProteinModelPortaliA5W9I5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A5W9I5-1 [UniParc]FASTAAdd to Basket

« Hide

MTTVQEAVPN LIPTQDATPR PAPKKVEAGV KLRGADKVAR IPVKIIPTDE    50
LPKKPDWIRV RIPVSPEVDR IKQLLRKHKL HSVCEEASCP NLGECFSGGT 100
ATFMIMGDIC TRRCPFCDVG HGRPKPLDLD EPKNLAVAIA DLRLKYVVIT 150
SVDRDDLRDG GAQHFADCIR EIRALSPGVQ LETLVPDYRG RMDVALEITA 200
QEPPDVFNHN LETVPRLYKA ARPGSDYDWS LDLLQKFKQL VPHVPTKSGL 250
MLGLGETDEE VIEVMHRMRE HDIDMLTLGQ YLQPSRSHLP VQRFVHPDTF 300
AWFAEEGYKM GFKNVASGPL VRSSYHADQQ AHEAKIKL 338
Length:338
Mass (Da):37,957
Last modified:July 10, 2007 - v1
Checksum:iCBCCB80702D3B749
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000712 Genomic DNA. Translation: ABQ80795.1.
RefSeqiYP_001269979.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ80795; ABQ80795; Pput_4675.
GeneIDi5195258.
KEGGippf:Pput_4675.
PATRICi19925594. VBIPsePut56420_4739.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000712 Genomic DNA. Translation: ABQ80795.1 .
RefSeqi YP_001269979.1. NC_009512.1.

3D structure databases

ProteinModelPortali A5W9I5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 351746.Pput_4675.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ80795 ; ABQ80795 ; Pput_4675 .
GeneIDi 5195258.
KEGGi ppf:Pput_4675.
PATRICi 19925594. VBIPsePut56420_4739.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci PPUT351746:GI26-4766-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiLIPA_PSEP1
AccessioniPrimary (citable) accession number: A5W9I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi