A5W8V4 (ALGL_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alginate lyase
EC=4.2.2.3

Alternative name(s):
Poly(beta-D-mannuronate) lyase
Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	Pput_4444

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond By similarity. HAMAP MF_00557
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Subcellular location	Periplasm Potential HAMAP MF_00557.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 367	343	Alginate lyase HAMAP MF_00557		PRO_5000252423

Sequences

	Sequence		Length	Mass (Da)
	A5W8V4 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: E96F1FD06AC75843	FASTA	367	40,947
	10 20 30 40 50 60 MTIFKRISSP ALLALALFGG AAHAALVPPQ GYYQGIEKLK TGEGNFRCEA APKPYTGPLQ 70 80 90 100 110 120 FRSKYEGSDK ARATLNAASE KAFRTSTEDI TTLEKGVSKM VGQYMRDGRP AQLDCTLTWL 130 140 150 160 170 180 GSWARAGALL STDYNHTGKS MRKWALGSMS GSWLRLKFSN SQPLAAHQAE ADLIEKWLSR 190 200 210 220 230 240 LAEQTVRDWS DLPLEKINNH SYWAAWSVMA TAVATDRRDL FDWAVKEYKV GANQVDDQGF 250 260 270 280 290 300 LPNEIKRKQR ALAYHNYALP PLAMIASFAQ ANGVDLRAEN NFALQRLGEG VLAGARDPSH 310 320 330 340 350 360 FKARAGKKQD MTDLKVDSKY SWLEPWCALY HCVGDTLERK HDMQPFNSFR LGGDVTRVYD PSAESKK

References

[1]

"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F1 / ATCC 700007.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000712 Genomic DNA. Translation: ABQ80564.1.
RefSeq	YP_001269748.1. NC_009512.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5W8V4.
Protein family/group databases
CAZy	PL5. Polysaccharide Lyase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5191334.
GenomeReviews	Gene locus Pput_4444 in contig CP000712_GR.
KEGG	ppf:Pput_4444.
PATRIC	19925111. VBIPsePut56420_4505.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG42597.
HOGENOM	HBG336064.
OMA	AAWSVMA.
ProtClustDB	PRK00325.
Enzyme and pathway databases
BioCyc	PPUT351746:PPUT_4444-MONOMER.
Family and domain databases
HAMAP	MF_00557. Alginate_lyase. [Tree]
InterPro	IPR022859. Alginate_lyase. IPR008397. Alginate_lyase_dom. IPR008929. Chondroitin_lyas. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
KO	K01729.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
SUPFAM	SSF48230. Chondroitin_lyas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGL_PSEP1

Accession

Primary (citable) accession number: A5W8V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program