A5W867 (PLSB_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Pput_4203

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 828	828	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049448
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A5W867 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 0416D77634A197B9	FASTA	828	93,590
	10 20 30 40 50 60 MTRSPLHRLI FGGLRRLLYL WVRSETINQS SMTLNLDRSR PVFYALPSPA LTDLAVLDHE 70 80 90 100 110 120 CTKAGLPRPV LPVAVGPLQE PAAFFYLTPD PDWLGRQDKS GAPPTLERLV AAVSQHAEED 130 140 150 160 170 180 AQIIPVSVFW GQTPASESSP WKLLFADSWA VTGRLRRLLT VLILGRKTRV QFSAPIHLRE 190 200 210 220 230 240 LVQHNKGHER TVRMAQRLMR VHFRNLKAAV IGPDISHRRT LVKGLVHAPQ VRQAIADEAQ 250 260 270 280 290 300 RENLPLAKAE AQALRYGNEI ASDYTYTAIR FLEVVLSWFW NKIYDGIKVN HIEQVQGIAP 310 320 330 340 350 360 GHEVIYVPCH RSHIDYLLLS YLLFRNGLTP PHVAAGINLN MPVVGNLLRR GGAFFMRRTF 370 380 390 400 410 420 KGNPLYTAVF NEYLHTLYTK GFPVEYFVEG GRSRTGRMLQ PRTGMLAITL RSFLRSSRTP 430 440 450 460 470 480 IVFVPVYIGY ERVLEGRTYL GELRGASKKK ESVLDIFKVF GALKQRFGQV YVNFGEPIRL 490 500 510 520 530 540 AGFLDQQQPG WREQDHGPQY RPDWLNAATA RLGETVARHL NEAAAINPVN LVALALLSTS 550 560 570 580 590 600 RLALDERALT RVLDLYLALL RQVPYSPHTT LPEGDGQALI EHVRSMNLVA EQKDALGRIL 610 620 630 640 650 660 YLDEGNAVLM TYYRNNVLHI FALPALLASF FLSSSRMSRE LLGQYVHALY PYLQAELFLR 670 680 690 700 710 720 WTPEQLDEVI DQWLVALVEQ GLLRQDNDLY VRPAPSSRQF VLLTLLARTI TQTLQRFYMA 730 740 750 760 770 780 TSLLINSGQN SLSAEALEDL CVMMAQRLSI LHGLNAPEFF DKTLFRHFIQ TLLQQGVLHA 790 800 810 820 DAQGKLGYHD KLGELAEGVA KRVLSAELRL SIRQVALHRD DGLETSTL

References

[1]

"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F1 / ATCC 700007.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000712 Genomic DNA. Translation: ABQ80327.1.
RefSeq	YP_001269511.1. NC_009512.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5W867.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5192554.
GenomeReviews	Gene locus Pput_4203 in contig CP000712_GR.
KEGG	ppf:Pput_4203.
PATRIC	19924619. VBIPsePut56420_4260.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	PPUT351746:PPUT_4203-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_PSEP1

Accession

Primary (citable) accession number: A5W867

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program