A5W852 (GLND_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
[Protein-PII] uridylyltransferase
Short name=PII uridylyl-transferase
EC=2.7.7.59

Alternative name(s):
UTase
Uridylyl-removing enzyme

Gene names

Name:	glnD
Ordered Locus Names:	Pput_4188

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (GlnB) regulatory protein By similarity. HAMAP-Rule MF_00277
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277
Sequence similarities	Belongs to the GlnD family.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: HAMAP
Molecular_function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 900	900	[Protein-PII] uridylyltransferase HAMAP-Rule MF_00277		PRO_1000059225

Sequences

	Sequence		Length	Mass (Da)
	A5W852 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 2DF98D1AF5A3B96B	FASTA	900	102,521
	10 20 30 40 50 60 MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRLAGEVLD KRFSAGSDIR PLIEARAWLV 70 80 90 100 110 120 DNILQQAWNQ FDWGDQSGIA LVAVGGYGRG ELHPHSDIDL LILLGAAEHE QYRDAIERFL 130 140 150 160 170 180 TLLWDIGLEV GQSVRTVDEC AEQARADLTV ITNLMESRTI AGPEALRQRM LEVTSTAHMW 190 200 210 220 230 240 PSKEFFLAKR AELKARHHKY NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QYGTLNLHAL 250 260 270 280 290 300 AGEGFLLESE NELLASSQDF LWKVRYALHM LAGRAEDRLL FDHQRSIATL LGYSDENPKR 310 320 330 340 350 360 AIEQFMQQYY RVVMSISQLC DLIIQHFEEV ILADEESGST QPLNARFRLH DGYIEAAHPN 370 380 390 400 410 420 VFKRTPFAML EIFVLMAQHP EIKGVRADTV RLLREHRHLI DDTFRTDIRN TSLFIELFKC 430 440 450 460 470 480 EIGIHRNLRR MNRYGILGRY LPEFGLIVGQ MQHDLFHIYT VDAHTLNLIK HLRKLQYTPV 490 500 510 520 530 540 SEKFPLASKL MGRLPKPELI YLAGLYHDIG KGRQGDHSEI GAVDAQKFCE RHQLPAWDSR 550 560 570 580 590 600 LIVWLVQNHL VMSTTAQRKD LSDPQVINDF ALHVGDETRL DYLYVLTVAD INATNPSLWN 610 620 630 640 650 660 SWRASLLRQL YTETKRALRR GLENPLDREE QIRQTQSSAL DILVREGTDP DDVEQLWAQL 670 680 690 700 710 720 GDDYFLKHTA ADVAWHTDAI LQQPADGGPL VLIKETTQRE FEGGTQIFIY APDQHDFFAV 730 740 750 760 770 780 TVAAMSQLNL NIHDARIITS SSQFTLDTYI VLDNDGGSIG DNPQRVKQIR DGLTEALRNP 790 800 810 820 830 840 EDYPTIIQRR VPRQLKHFDF PPQVTILNDA QRPVTILEIT APDRPGLLAR LGRIFLEFDL 850 860 870 880 890 900 SLQNAKIATL GERVEDVFFI TDADNQPLSD PQLCSRLQEA IVQQLQAGQG SDTSQTRVTF

References

[1]

"Complete sequence of Pseudomonas putida F1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F1 / ATCC 700007.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000712 Genomic DNA. Translation: ABQ80312.1.
RefSeq	YP_001269496.1. NC_009512.1.
3D structure databases
ProteinModelPortal	A5W852.
ModBase	Search...
Protein-protein interaction databases
STRING	351746.Pput_4188.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ80312; ABQ80312; Pput_4188.
GeneID	5192565.
KEGG	ppf:Pput_4188.
PATRIC	19924589. VBIPsePut56420_4245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2844.
HOGENOM	HOG000261778.
KO	K00990.
OMA	SPKVWNA.
ProtClustDB	PRK00275.
Enzyme and pathway databases
BioCyc	PPUT351746:GI26-4283-MONOMER.
Family and domain databases
Gene3D	1.10.3210.10. 1 hit.
HAMAP	MF_00277. PII_uridylyl-transf.
InterPro	IPR002912. ACT_dom. IPR010043. GlnD_Uridyltrans. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. PTHR13734:SF1. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_PSEP1

Accession

Primary (citable) accession number: A5W852

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program