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Protein

Protein-L-isoaspartate O-methyltransferase

Gene

pcm

Organism
Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPUT351746:GI26-4239-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate O-methyltransferaseUniRule annotation (EC:2.1.1.77UniRule annotation)
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferaseUniRule annotation
Protein L-isoaspartyl methyltransferaseUniRule annotation
Protein-beta-aspartate methyltransferaseUniRule annotation
Short name:
PIMTUniRule annotation
Gene namesi
Name:pcmUniRule annotation
Ordered Locus Names:Pput_4156
OrganismiPseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006553 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Protein-L-isoaspartate O-methyltransferasePRO_0000351910Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_4156.

Structurei

3D structure databases

ProteinModelPortaliA5W820.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E26. Bacteria.
COG2518. LUCA.
HOGENOMiHOG000257189.
KOiK00573.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5W820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSQRTRERL IQRLCEEGVS NTKVLDVIRR TPRHLFVDEA LAHRAYEDTA
60 70 80 90 100
LPIGHNQTIS QPFMVAHMSE LLLEAGPLDK VLEIGTGSGY QTAILAQLVE
110 120 130 140 150
RVFSVERIKV LQDRAKERLV ELNLRNVVFR WGDGCEGWPA LAPYNGIIVT
160 170 180 190 200
AVAPEVPQAL LDQLAPGGRM VIPVGPAGEA QQLMLIVREE HGFSRRVLGA
210
VRFVPLLNGP LA
Length:212
Mass (Da):23,469
Last modified:October 14, 2008 - v2
Checksum:iF9E929C5BFDB3E4D
GO

Sequence cautioni

The sequence ABQ80280 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ80280.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiABQ80280; ABQ80280; Pput_4156.
KEGGippf:Pput_4156.
PATRICi19924525. VBIPsePut56420_4213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ80280.1. Different initiation.

3D structure databases

ProteinModelPortaliA5W820.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_4156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ80280; ABQ80280; Pput_4156.
KEGGippf:Pput_4156.
PATRICi19924525. VBIPsePut56420_4213.

Phylogenomic databases

eggNOGiENOG4105E26. Bacteria.
COG2518. LUCA.
HOGENOMiHOG000257189.
KOiK00573.

Enzyme and pathway databases

BioCyciPPUT351746:GI26-4239-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIMT_PSEP1
AccessioniPrimary (citable) accession number: A5W820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: October 14, 2008
Last modified: September 7, 2016
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.