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Protein

Adenosylcobinamide-GDP ribazoletransferase

Gene

cobS

Organism
Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.UniRule annotation

Catalytic activityi

Adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin.UniRule annotation
Adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate = GMP + adenosylcobalamin 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPPUT351746:GI26-4121-MONOMER.
UniPathwayiUPA00148; UER00238.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylcobinamide-GDP ribazoletransferaseUniRule annotation (EC:2.7.8.26UniRule annotation)
Alternative name(s):
Cobalamin synthaseUniRule annotation
Cobalamin-5'-phosphate synthaseUniRule annotation
Gene namesi
Name:cobSUniRule annotation
Ordered Locus Names:Pput_4038
OrganismiPseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006553 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 5121HelicalUniRule annotationAdd
BLAST
Transmembranei62 – 8120HelicalUniRule annotationAdd
BLAST
Transmembranei109 – 12921HelicalUniRule annotationAdd
BLAST
Transmembranei133 – 15321HelicalUniRule annotationAdd
BLAST
Transmembranei179 – 19921HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Adenosylcobinamide-GDP ribazoletransferasePRO_1000062092Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_4038.

Family & Domainsi

Sequence similaritiesi

Belongs to the CobS family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GN9. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
KOiK02233.
OMAiAWLRHLM.

Family and domain databases

HAMAPiMF_00719. CobS. 1 hit.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.

Sequencei

Sequence statusi: Complete.

A5W7Q2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPFWIALQF LSSLPVSLPG MPAPREVGRS LLYYPLVGLL FGLLLWLASH
60 70 80 90 100
LLQGTPSPLH AALLLTLWVL LSGALHLDGL ADSADAWLGG FGDRERTLRI
110 120 130 140 150
MKDPRSGPIA VVTLVLVLLL KFCALWVLVG QGIGAQLLLA PLIGRAAMLG
160 170 180 190 200
LFLGTPYVRP GGLGQALAEH LPRRAAGWVL LVCVLFCLFL GGWSVLLALA
210 220 230 240
VFAWLRHLMC RRLGGTTGDT AGALLELLEL AVVLGLALGL
Length:240
Mass (Da):25,587
Last modified:July 10, 2007 - v1
Checksum:i76A3BCFA27B92EC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ80162.1.
RefSeqiWP_004575325.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ80162; ABQ80162; Pput_4038.
KEGGippf:Pput_4038.
PATRICi19924301. VBIPsePut56420_4101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ80162.1.
RefSeqiWP_004575325.1. NC_009512.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_4038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ80162; ABQ80162; Pput_4038.
KEGGippf:Pput_4038.
PATRICi19924301. VBIPsePut56420_4101.

Phylogenomic databases

eggNOGiENOG4105GN9. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
KOiK02233.
OMAiAWLRHLM.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00238.
BioCyciPPUT351746:GI26-4121-MONOMER.

Family and domain databases

HAMAPiMF_00719. CobS. 1 hit.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOBS_PSEP1
AccessioniPrimary (citable) accession number: A5W7Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: September 7, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.