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Protein

Glutaminase

Gene

glsA

Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611SubstrateUniRule annotation
Binding sitei111 – 1111SubstrateUniRule annotation
Binding sitei155 – 1551SubstrateUniRule annotation
Binding sitei162 – 1621SubstrateUniRule annotation
Binding sitei186 – 1861SubstrateUniRule annotation
Binding sitei238 – 2381SubstrateUniRule annotation
Binding sitei256 – 2561Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciPPUT351746:GI26-3245-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GlutaminaseUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:glsAUniRule annotation
Ordered Locus Names:Pput_3197
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006553 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302GlutaminasePRO_1000048344Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi351746.Pput_3197.

Structurei

3D structure databases

ProteinModelPortaliA5W5B3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutaminase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
KOiK01425.
OMAiVNSIMAT.
OrthoDBiEOG6N94BK.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

Sequencei

Sequence statusi: Complete.

A5W5B3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTLLNEILE EVRPLIGKGK VADYIPALAD VPANQLGIAV YGNDGSYHCA
60 70 80 90 100
GDARVPFSVQ SISKVFSLVQ AIGHSGEAIW ERLGHEPSGQ PFNSLVQLEF
110 120 130 140 150
ERGRPRNPFI NAGALVICDI NQSRFAAPTL SMRDFVRRLS GNPHISINTR
160 170 180 190 200
VADSEYQFRA RNAAMAYLMQ SFGNFHNEVE TVLRSYFSYC ALQMNCLDLA
210 220 230 240 250
RAFCFLANDG FCKHSGEQIL TRRQTQQVNS IMATSGLYDE AGNFAFRVGL
260 270 280 290 300
PGKSGVGGGI VAIVPGQFTV CVWSPELNAA GNSLAGMAAL ELLSSRIGWS

VF
Length:302
Mass (Da):32,875
Last modified:July 10, 2007 - v1
Checksum:i1028042472EC1652
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ79323.1.
RefSeqiWP_004575383.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ79323; ABQ79323; Pput_3197.
KEGGippf:Pput_3197.
PATRICi19922535. VBIPsePut56420_3252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ79323.1.
RefSeqiWP_004575383.1. NC_009512.1.

3D structure databases

ProteinModelPortaliA5W5B3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_3197.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ79323; ABQ79323; Pput_3197.
KEGGippf:Pput_3197.
PATRICi19922535. VBIPsePut56420_3252.

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
KOiK01425.
OMAiVNSIMAT.
OrthoDBiEOG6N94BK.

Enzyme and pathway databases

BioCyciPPUT351746:GI26-3245-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiGLSA_PSEP1
AccessioniPrimary (citable) accession number: A5W5B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: July 22, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.