Reviewed, UniProtKB/Swiss-Prot A5W4G5 (CMTB_PSEP1)

Names and originHide

Protein names

Recommended name:
2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase
EC=1.3.1.58

Alternative name(s):
Biphenyl-2,3-dihydro-2,3-diol dehydrogenase

Gene names

Name:	cmtB
Ordered Locus Names:	Pput_2895

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributesHide

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Catalytic activity	Cis-5,6-dihydroxy-4-isopropylcyclohexa-1,3-dienecarboxylate + NAD+ = 2,3-dihydroxy-p-cumate + NADH.
Pathway	Aromatic compound metabolism; p-cumate degradation; acetaldehyde and pyruvate from p-cumate: step 2/7.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

OntologiesHide

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase		PRO_0000314469
Regions
	Nucleotide binding	18 – 42	25	NAD By similarity
Sites
	Active site	163	1	Proton acceptor By similarity

SequencesHide

	Sequence		Length	Mass (Da)
	A5W4G5-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: B4CD9C3DDC833A8B	FASTA	259	27,310
	10 20 30 40 50 60 MSTLSRNALP LEGQVAVVTG GAHGIGLGIV ERLLGLGARV TASDIDESGL SLLCERLAAK 70 80 90 100 110 120 HADAIAVHAA DLSEEQGAQG LHRAAVERFG SVQILVNCAG GGVIRPFLEH TPETLKATID 130 140 150 160 170 180 RNLWTALWCS RVFLPDMLAR QYGRIINIGA DSVRNGLPDH AAYNAAKGGM HGLTTGLARE 190 200 210 220 230 240 FARQGVTVNT VAPCAVNTEV WVRIKNANPE LAQRFLDVIP MGRVGEIEEV ASMVGYLAQP 250 EAAFVTGQVI SVNGGSTML

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"p-cumate catabolic pathway in Pseudomonas putida F1: cloning and characterization of DNA carrying the cmt operon." Eaton R.W. J. Bacteriol. 178:1351-1362(1996) [PubMed: 8631713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	U24215 Genomic DNA. Translation: AAB62288.1. CP000712 Genomic DNA. Translation: ABQ79025.1.
RefSeq	YP_001268209.1.
3D structure databases
SMR	A5W4G5. Positions 10-258.
ModBase	Search...
Protein-protein interaction databases
STRING	A5W4G5.
Genome annotation databases
GeneID	5191657.
GenomeReviews	Gene locus Pput_2895 in contig CP000712_GR.
KEGG	ppf:Pput_2895.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1028.
HOGENOM	HBG750976.
OMA	CAVNTEV.
ProtClustDB	CLSK845951.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

CMTB_PSEP1

Accession

Primary (citable) accession number: A5W4G5
Secondary accession number(s): Q51977

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	July 13, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)