Benzene 1,2-dioxygenase subunit alpha - Pseudomonas putida (strain F1 / ATCC 700007)

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Reviewed, UniProtKB/Swiss-Prot A5W4F2 (BNZA_PSEP1)

Last modified November 25, 2008. Version 13. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Benzene 1,2-dioxygenase subunit alpha
    EC=1.14.12.3
Alternative name(s):
    Benzene 1,2-dioxygenase P1 subunit
    Toluene 2,3-dioxygenase subunit alpha
    EC=1.14.12.11

Gene names

Name:	bnzA
Synonyms:	todC1
Ordered Locus Names:	Pput_2881

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes both the oxidation of benzene and toluene.
Catalytic activity	Benzene + NADH + O(2) = cis-cyclohexa-3,5-diene-1,2-diol + NAD(+). Toluene + NADH + O(2) = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD(+).
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 1 iron ion per subunit By similarity.
Pathway	Aromatic compound metabolism; benzene degradation; catechol from benzene: step 1/2. Xenobiotic degradation; toluene degradation. Xenobiotic degradation; xylene degradation.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (bnzA and bnzB), a ferredoxin (bnzC) and a ferredoxin reductase (bnzD).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain.

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Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro benzene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW toluene dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 450

450

Benzene 1,2-dioxygenase subunit alpha

PRO_0000314465

Regions

Domain

54 – 163

110

Rieske

Sites

Metal binding

Iron-sulfur (2Fe-2S) By similarity

Metal binding

Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Metal binding

116

Iron-sulfur (2Fe-2S) By similarity

Metal binding

119

Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Metal binding

222

Iron By similarity

Metal binding

228

Iron By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

A5W4F2-1 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 038C80F197F3485D
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FASTA

450

50,944

        10         20         30         40         50         60 
MNQTDTSPIR LRRSWNTSEI EALFDEHAGR IDPRIYTDED LYQLELERVF ARSWLLLGHE 

        70         80         90        100        110        120 
TQIRKPGDYI TTYMGEDPVV VVRQKDASIA VFLNQCRHRG MRICRADAGN AKAFTCSYHG 

       130        140        150        160        170        180 
WAYDTAGNLV NVPYEAESFA CLNKKEWSPL KARVETYKGL IFANWDENAV DLDTYLGEAK 

       190        200        210        220        230        240 
FYMDHMLDRT EAGTEAIPGV QKWVIPCNWK FAAEQFCSDM YHAGTTSHLS GILAGLPEDL 

       250        260        270        280        290        300 
EMADLAPPTV GKQYRASWGG HGSGFYVGDP NLMLAIMGPK VTSYWTEGPA SEKAAERLGS 

       310        320        330        340        350        360 
VERGSKLMVE HMTVFPTCSF LPGINTVRTW HPRGPNEVEV WAFTVVDADA PDDIKEEFRR 

       370        380        390        400        410        420 
QTLRTFSAGG VFEQDDGENW VEIQHILRGH KARSRPFNAE MSMDQTVDND PVYPGRISNN 

       430        440        450 
VYSEEAARGL YAHWLRMMTS PDWDALKATR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli." Zylstra G.J., Gibson D.T. J. Biol. Chem. 264:14940-14946(1989) [PubMed: 2670929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
[2]	"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	J04996 Genomic DNA. Translation: AAA26005.1. CP000712 Genomic DNA. Translation: ABQ79012.1.
PIR	A36516.
RefSeq	YP_001268196.1.
3D structure databases
HSSP	HSSP built from PDB template 1O7N based on UniProtKB P23094.
ModBase	Search...
Genome annotation databases
GeneID	5195399.
GenomeReviews	Gene locus Pput_2881 in contig CP000712_GR.
KEGG	ppf:Pput_2881.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR005806. Rieske_reg. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. IPR015879. Ring_hydroxy_dOase_asu_C. IPR001663. Rng_hydr_dOase-A. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR21266:SF2. Rng_hydr_dOase-A. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF00848. Ring_hydroxyl_A. 1 hit. [Graphical view]
PRINTS	PR00090. RNGDIOXGNASE.
PROSITE	PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

BNZA_PSEP1

Accession

Primary (citable) accession number: A5W4F2
Secondary accession number(s): P08084, P13450

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Family and domain databases

Entry information

Relevant documents