ID   BNZB_PSEP1              Reviewed;         187 AA.
AC   A5W4F1; P08085; P13451;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   26-MAY-2009, entry version 12.
DE   RecName: Full=Benzene 1,2-dioxygenase subunit beta;
DE            EC=1.14.12.3;
DE   AltName: Full=Benzene 1,2-dioxygenase P2 subunit;
DE   AltName: Full=Toluene 2,3-dioxygenase subunit beta;
DE            EC=1.14.12.11;
GN   Name=bnzB; Synonyms=todC2; OrderedLocusNames=Pput_2880;
OS   Pseudomonas putida (strain F1 / ATCC 700007).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-12.
RX   MEDLINE=89359301; PubMed=2670929;
RA   Zylstra G.J., Gibson D.T.;
RT   "Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of
RT   the todC1C2BADE genes and their expression in Escherichia coli.";
RL   J. Biol. Chem. 264:14940-14946(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Parales R.,
RA   Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes both the oxidation of benzene and toluene. The
CC       beta subunit may be responsible for the substrate specificity of
CC       the enzyme.
CC   -!- CATALYTIC ACTIVITY: Benzene + NADH + O(2) = cis-cyclohexa-3,5-
CC       diene-1,2-diol + NAD(+).
CC   -!- CATALYTIC ACTIVITY: Toluene + NADH + O(2) = (1S,2R)-3-
CC       methylcyclohexa-3,5-diene-1,2-diol + NAD(+).
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 iron ion per subunit (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; benzene degradation;
CC       catechol from benzene: step 1/2.
CC   -!- PATHWAY: Xenobiotic degradation; toluene degradation.
CC   -!- PATHWAY: Xenobiotic degradation; xylene degradation.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the
CC       two subunits of the hydroxylase component (bnzA and bnzB), a
CC       ferredoxin (bnzC) and a ferredoxin reductase (bnzD).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase beta subunit family.
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DR   EMBL; J04996; AAA26006.1; -; Genomic_DNA.
DR   EMBL; CP000712; ABQ79011.1; -; Genomic_DNA.
DR   PIR; B29830; B29830.
DR   RefSeq; YP_001268195.1; -.
DR   PDB; 3EN1; X-ray; 3.20 A; B=1-187.
DR   PDB; 3EQQ; X-ray; 3.20 A; B=1-187.
DR   PDBsum; 3EN1; -.
DR   PDBsum; 3EQQ; -.
DR   GeneID; 5195398; -.
DR   GenomeReviews; CP000712_GR; Pput_2880.
DR   KEGG; ppf:Pput_2880; -.
DR   OMA; A5W4F1; DIFAGER.
DR   GO; GO:0018619; F:benzene 1,2-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0018624; F:toluene dioxygenase activity; IEA:EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aromatic hydrocarbons catabolism; Complete proteome;
KW   Dioxygenase; Direct protein sequencing; NAD; Oxidoreductase.
FT   CHAIN         1    187       Benzene 1,2-dioxygenase subunit beta.
FT                                /FTId=PRO_0000314466.
SQ   SEQUENCE   187 AA;  22013 MW;  39A5D2BECB6116B5 CRC64;
     MIDSANRADV FLRKPAPVAP ELQHEVEQFY YWEAKLLNDR RFEEWFALLA EDIHYFMPIR
     TTRIMRDSRL EYSGSREYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HLVSNVMIVG
     AEAEGEYEIS SAFIVYRNRL ERQLDIFAGE RRDTLRRNTS EAGFEIVNRT ILIDQSTILA
     NNLSFFF
//