Reviewed, UniProtKB/Swiss-Prot A5W4F1 (BNZB_PSEP1)

Names and origin

Protein names

Recommended name:
    Benzene 1,2-dioxygenase subunit beta
    EC=1.14.12.3
Alternative name(s):
    Benzene 1,2-dioxygenase P2 subunit
    Toluene 2,3-dioxygenase subunit beta
    EC=1.14.12.11

Gene names

Name:	bnzB
Synonyms:	todC2
Ordered Locus Names:	Pput_2880

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes both the oxidation of benzene and toluene. The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Benzene + NADH + O(2) = cis-cyclohexa-3,5-diene-1,2-diol + NAD(+). Toluene + NADH + O(2) = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD(+).
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 1 iron ion per subunit By similarity.
Pathway	Aromatic compound metabolism; benzene degradation; catechol from benzene: step 1/2. Xenobiotic degradation; toluene degradation. Xenobiotic degradation; xylene degradation.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (bnzA and bnzB), a ferredoxin (bnzC) and a ferredoxin reductase (bnzD).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	benzene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW toluene dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Benzene 1,2-dioxygenase subunit beta		PRO_0000314466

Sequences

	Sequence		Length	Mass (Da)
	A5W4F1-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 39A5D2BECB6116B5	FASTA	187	22,013
	10 20 30 40 50 60 MIDSANRADV FLRKPAPVAP ELQHEVEQFY YWEAKLLNDR RFEEWFALLA EDIHYFMPIR 70 80 90 100 110 120 TTRIMRDSRL EYSGSREYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HLVSNVMIVG 130 140 150 160 170 180 AEAEGEYEIS SAFIVYRNRL ERQLDIFAGE RRDTLRRNTS EAGFEIVNRT ILIDQSTILA NNLSFFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli." Zylstra G.J., Gibson D.T. J. Biol. Chem. 264:14940-14946(1989) [PubMed: 2670929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
[2]	"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04996 Genomic DNA. Translation: AAA26006.1. CP000712 Genomic DNA. Translation: ABQ79011.1.
PIR	B29830.
RefSeq	YP_001268195.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5195398.
GenomeReviews	Gene locus Pput_2880 in contig CP000712_GR.
KEGG	ppf:Pput_2880.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR000391. Rng_hydr_dOase-B. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BNZB_PSEP1

Accession

Primary (citable) accession number: A5W4F1
Secondary accession number(s): P08085, P13451

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	November 4, 2008
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)