A5W4F1 (BNZB_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Benzene 1,2-dioxygenase subunit beta
EC=1.14.12.3

Alternative name(s):
Benzene 1,2-dioxygenase P2 subunit
Toluene 2,3-dioxygenase subunit beta
EC=1.14.12.11

Gene names

Name:	bnzB
Synonyms:	todC2
Ordered Locus Names:	Pput_2880

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes both the oxidation of benzene and toluene. The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Benzene + NADH + O2 = cis-cyclohexa-3,5-diene-1,2-diol + NAD+. Toluene + NADH + O2 = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 1 iron ion per subunit By similarity.
Pathway	Aromatic compound metabolism; benzene degradation; catechol from benzene: step 1/2. Xenobiotic degradation; toluene degradation. Xenobiotic degradation; xylene degradation.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (BnzA and BnzB), a ferredoxin (BnzC) and a ferredoxin reductase (BnzD).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	benzene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW toluene dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 187

187

Benzene 1,2-dioxygenase subunit beta

PRO_0000314466

Secondary structure

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187

Helix Strand Turn

Details...

Helix

10 – 12

3

Helix

20 – 38

19

Helix

42 – 47

6

Beta strand

49 – 58

10

Helix

65 – 70

6

Beta strand

79 – 83

5

Helix

85 – 88

4

Turn

92 – 95

4

Helix

101 – 103

3

Beta strand

108 – 119

12

Beta strand

127 – 139

13

Turn

140 – 142

3

Beta strand

143 – 147

5

Beta strand

151 – 156

6

Beta strand

165 – 171

7

Beta strand

176 – 181

6

Sequences

	Sequence		Length	Mass (Da)
	A5W4F1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 39A5D2BECB6116B5	FASTA	187	22,013
	10 20 30 40 50 60 MIDSANRADV FLRKPAPVAP ELQHEVEQFY YWEAKLLNDR RFEEWFALLA EDIHYFMPIR 70 80 90 100 110 120 TTRIMRDSRL EYSGSREYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HLVSNVMIVG 130 140 150 160 170 180 AEAEGEYEIS SAFIVYRNRL ERQLDIFAGE RRDTLRRNTS EAGFEIVNRT ILIDQSTILA NNLSFFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli." Zylstra G.J., Gibson D.T. J. Biol. Chem. 264:14940-14946(1989) [PubMed: 2670929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
[2]	"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F1 / ATCC 700007.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J04996 Genomic DNA. Translation: AAA26006.1.
CP000712 Genomic DNA. Translation: ABQ79011.1.

PIR

B29830.

RefSeq

YP_001268195.1. NC_009512.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EN1	X-ray	3.20	B	1-187	[»]
3EQQ	X-ray	3.20	B	1-187	[»]

ProteinModelPortal

A5W4F1.

SMR

A5W4F1. Positions 11-187.

ModBase

Search...

Protein-protein interaction databases

STRING

A5W4F1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

5195398.

GenomeReviews

Gene locus Pput_2880 in contig CP000712_GR.

KEGG

ppf:Pput_2880.

PATRIC

19921903. VBIPsePut56420_2936.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG5517.

HOGENOM

HBG582498.

OMA

DIFAGER.

ProtClustDB

CLSK922463.

Enzyme and pathway databases

BioCyc

PPUT351746:PPUT_2880-MONOMER.

Family and domain databases

InterPro

IPR000391. Rng_hydr_dOase-bsu.
[Graphical view]

KO

K08689.

Pfam

PF00866. Ring_hydroxyl_B. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BNZB_PSEP1

Accession

Primary (citable) accession number: A5W4F1
Secondary accession number(s): P08085, P13451

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program