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A5W4E9 (TODA_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Toluene 1,2-dioxygenase system ferredoxin--NAD(+) reductase component
EC=1.18.1.3
Gene names
Name:todA
Ordered Locus Names:Pput_2878
OrganismPseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]
Taxonomic identifier351746 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the electron transfer component of toluene 1,2-dioxygenase, transfers electrons from ferredoxin (todB) to NADH.

Catalytic activity

Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.

Cofactor

FAD.

Pathway

Xenobiotic degradation; toluene degradation.

Subunit structure

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (todC1 and todC2), a ferredoxin (todB) and a ferredoxin reductase (todA).

Sequence similarities

Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 410409Toluene 1,2-dioxygenase system ferredoxin--NAD(+) reductase component
PRO_0000314468

Regions

Nucleotide binding4 – 3532FAD Potential
Nucleotide binding145 – 17329NAD Potential

Secondary structure

1 ............................................................ 410
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
A5W4E9 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 934CF2EDD2AB1450

FASTA41042,942
        10         20         30         40         50         60 
MATHVAIIGN GVGGFTTAQA LRAEGFEGRI SLIGDEPHLP YDRPSLSKAV LDGSLERPPI 

        70         80         90        100        110        120 
LAEADWYGEA RIDMLTGPEV TALDVQTRTI SLDDGTTLSA DAIVIATGSR ARTMALPGSQ 

       130        140        150        160        170        180 
LPGVVTLRTY GDVQVLRDSW TSATRLLIVG GGLIGCEVAT TARKLGLSVT ILEAGDELLV 

       190        200        210        220        230        240 
RVLGRRIGAW LRGLLTELGV QVELGTGVVG FSGEGQLEQV MASDGRSFVA DSALICVGAE 

       250        260        270        280        290        300 
PADQLARQAG LACDRGVIVD HCGATLAKGV FAVGDVASWP LRAGGRRSLE TYMNAQRQAA 

       310        320        330        340        350        360 
AVAAAILGKN VSAPQLPVSW TEIAGHRMQM AGDIEGPGDF VSRGMPGSGA ALLFRLQERR 

       370        380        390        400        410 
IQAVVAVDAP RDFALATRLV EARAAIEPAR LADLSNSMRD FVRANEGDLT

« Hide

References

« Hide 'large scale' references
[1]"Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli."
Zylstra G.J., Gibson D.T.
J. Biol. Chem. 264:14940-14946(1989) [PubMed: 2670929] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13.
[2]"Complete sequence of Pseudomonas putida F1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F1 / ATCC 700007.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J04996 Genomic DNA. Translation: AAA26008.1.
CP000712 Genomic DNA. Translation: ABQ79009.1.
PIRD36516.
RefSeqYP_001268193.1. NC_009512.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3EF6X-ray1.80A1-410[»]
ProteinModelPortalA5W4E9.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5W4E9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5192603.
GenomeReviewsGene locus Pput_2878 in contig CP000712_GR.
KEGGppf:Pput_2878.
PATRIC19921899. VBIPsePut56420_2934.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0446.
HOGENOMHBG644911.
OMACEVATTA.
ProtClustDBCLSK922465.

Enzyme and pathway databases

BioCycPPUT351746:PPUT_2878-MONOMER.

Family and domain databases

InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
Gene3DG3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KOK00529.
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTODA_PSEP1
AccessionPrimary (citable) accession number: A5W4E9
Secondary accession number(s): P13452
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents