A5W499 (UPPP_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Undecaprenyl-diphosphatase
EC=3.6.1.27

Alternative name(s):
Bacitracin resistance protein
Undecaprenyl pyrophosphate phosphatase

Gene names

Name:	uppP
Ordered Locus Names:	Pput_2827

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin By similarity. HAMAP-Rule MF_01006
Catalytic activity	Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate. HAMAP-Rule MF_01006
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01006.
Miscellaneous	Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available. HAMAP-Rule MF_01006
Sequence similarities	Belongs to the UppP family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dephosphorylation Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	undecaprenyl-diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Undecaprenyl-diphosphatase HAMAP-Rule MF_01006		PRO_1000062809
Regions
	Transmembrane	43 – 63	21	Helical; Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Transmembrane	214 – 234	21	Helical; Potential
	Transmembrane	249 – 269	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A5W499 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 93D49BB9C69797BF	FASTA	276	30,621
	10 20 30 40 50 60 MDFWTAFQAI ILGVVEGLTE FLPISSTGHQ IIVADLIGFG GERAMAFNII IQLAAILAVV 70 80 90 100 110 120 WEFRSKIFEV VFGLTHQPKA RRFTGNLLLA FMPAVVLGVL FADLIHEYLF NPVTVAAALV 130 140 150 160 170 180 VGGVIMLWAE RRKHRVEVDH VDDMRWSHAL KIGFIQCLAM IPGTSRSGST IIGGLLFGLS 190 200 210 220 230 240 RKAATEFSFF LAMPTMVGAA VYSGYKYRDL FQPGDLPVFA LGFVTSFIFA MIAVRALLKF 250 260 270 IANHSYAAFA WYRIVFGLFI LATWQFGWVD WSTAHG

References

[1]

"Complete sequence of Pseudomonas putida F1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F1 / ATCC 700007.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000712 Genomic DNA. Translation: ABQ78959.1.
RefSeq	YP_001268143.1. NC_009512.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	351746.Pput_2827.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ78959; ABQ78959; Pput_2827.
GeneID	5194279.
KEGG	ppf:Pput_2827.
PATRIC	19921797. VBIPsePut56420_2883.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1968.
HOGENOM	HOG000218356.
KO	K06153.
OMA	RWLLRYI.
ProtClustDB	PRK00281.
Enzyme and pathway databases
BioCyc	PPUT351746:GI26-2922-MONOMER.
Family and domain databases
HAMAP	MF_01006. Undec_diphosphatase.
InterPro	IPR003824. Bacitracin-R_BacA. [Graphical view]
Pfam	PF02673. BacA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00753. undec_PP_bacA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPPP_PSEP1

Accession

Primary (citable) accession number: A5W499

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program