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A5W1H7

- CYSG_PSEP1

UniProt

A5W1H7 - CYSG_PSEP1

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Protein

Siroheme synthase

Gene

cysG

Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei248 – 2481Proton acceptorUniRule annotation
Active sitei270 – 2701Proton donorUniRule annotation
Binding sitei306 – 3061S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei383 – 3831S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei412 – 4121S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NADUniRule annotation
Nucleotide bindingi43 – 442NADUniRule annotation

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPUT351746:GI26-1880-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferaseUniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylaseUniRule annotation
Alternative name(s):
SUMTUniRule annotation
Uroporphyrinogen III methylaseUniRule annotation
Short name:
UROMUniRule annotation
Precorrin-2 dehydrogenaseUniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelataseUniRule annotation (EC:4.99.1.4UniRule annotation)
Gene namesi
Name:cysGUniRule annotation
Ordered Locus Names:Pput_1834
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006553: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Siroheme synthasePRO_0000330540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281PhosphoserineUniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_1834.

Structurei

3D structure databases

ProteinModelPortaliA5W1H7.
SMRiA5W1H7. Positions 1-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
BLAST
Regioni216 – 463248Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
BLAST
Regioni301 – 3033S-adenosyl-L-methionine bindingUniRule annotation
Regioni331 – 3322S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiQASFIMP.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5W1H7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYLPLFHKL QGGRVLVVGG GEIALRKARL LADAGGVLRV VAPDVDGQLA
60 70 80 90 100
ALAREGGGEV LVRGYQAADL VGCRLVIAAT DDPGLNAQVS ADAQALSVPV
110 120 130 140 150
NVVDAPALCT VIFPAIVDRS PLVIAVSSGG DAPVLARLIR AKLEAWIPSA
160 170 180 190 200
YGELAGLAAR FRHKVKSLYP DVNQRRGFWE TVFQGPIAER QLAGQGAEAE
210 220 230 240 250
RLLQAMVDGA PVQQGGEVYL VGAGPGDPDL LTFRALRLMQ QADVVLYDRL
260 270 280 290 300
VAPAIIDMCR RDAERIYVGK RRADHSVPQD QINRLLVDLA RQGKRVLRLK
310 320 330 340 350
GGDPFIFGRG GEEIEELAEH GIPFQVVPGI TAASGCSAYG GIPLTHRDYA
360 370 380 390 400
QSVRFVTGHL KDGTSNLPWT DLVAPAQTLV FYMGLVGLPT ICAELIRHGR
410 420 430 440 450
AASTPAALVQ QGTTRNQRVF TGTLADLPDL VAQHEVHAPT LVIVGEVVQL
460
RDKLAWFEGS QNS
Length:463
Mass (Da):49,467
Last modified:July 10, 2007 - v1
Checksum:i7738ABD519697647
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ77987.1.
RefSeqiYP_001267171.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ77987; ABQ77987; Pput_1834.
GeneIDi5193098.
KEGGippf:Pput_1834.
PATRICi19919764. VBIPsePut56420_1867.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ77987.1 .
RefSeqi YP_001267171.1. NC_009512.1.

3D structure databases

ProteinModelPortali A5W1H7.
SMRi A5W1H7. Positions 1-459.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 351746.Pput_1834.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ77987 ; ABQ77987 ; Pput_1834 .
GeneIDi 5193098.
KEGGi ppf:Pput_1834.
PATRICi 19919764. VBIPsePut56420_1867.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi QASFIMP.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci PPUT351746:GI26-1880-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiCYSG_PSEP1
AccessioniPrimary (citable) accession number: A5W1H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 10, 2007
Last modified: October 1, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3