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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi252Magnesium or manganese 1UniRule annotation1
Metal bindingi264Magnesium or manganese 1UniRule annotation1
Metal bindingi264Magnesium or manganese 2UniRule annotation1
Metal bindingi266Magnesium or manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT351746:G1G90-1594-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC:6.3.2.4UniRule annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:Pput_1516Imported
OrganismiPseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)Imported
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006553 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_1516

Structurei

3D structure databases

ProteinModelPortaliA5W0L3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini102 – 297ATP-graspInterPro annotationAdd BLAST196

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011592
KOiK01921
OMAiRCDFFVI

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 2 hits
SUPFAMiSSF52440 SSF52440, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

A5W0L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIENIAIITG GNSSEREVSL QTSEAVSQSL KTLQISHEIF TISDYRELLS
60 70 80 90 100
LDLAKFTRVF LALHGGFGEN GMAQAYLEGL GIPYNGPSPQ ASAICMDKLL
110 120 130 140 150
TKHIARGLGI NVANYLYSKN GDDVCFEEIS ERLGTPCIVK PNREGCSFGV
160 170 180 190 200
SLVRDLKADL QPAINAAAKF HTGILIEEFI SGPELSVCYL NKSTLPIYTV
210 220 230 240 250
EFESDFFSYD AKFISTKTNA TLSILDRSTH QLVTNYCSAI AEALELDYFR
260 270 280 290 300
ADFIVHNTEP YLIELNTLPG LTSHSLFPKA CQQHGIEFDK LVLILNNLDP

SQYC
Length:304
Mass (Da):33,566
Last modified:July 10, 2007 - v1
Checksum:iD9D4706A47A914C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA Translation: ABQ77673.1
RefSeqiWP_012051638.1, NC_009512.1

Genome annotation databases

EnsemblBacteriaiABQ77673; ABQ77673; Pput_1516
KEGGippf:Pput_1516

Similar proteinsi

Entry informationi

Entry nameiA5W0L3_PSEP1
AccessioniPrimary (citable) accession number: A5W0L3
Entry historyiIntegrated into UniProtKB/TrEMBL: July 10, 2007
Last sequence update: July 10, 2007
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health