Reviewed, UniProtKB/Swiss-Prot A5W0D8 (ASTE_PSEP1)

Names and origin

Protein names

Recommended name:
    Succinylglutamate desuccinylase
    EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	Pput_1440

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the aspA/astE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_1000017326
Sites
	Active site	215	1	Potential
	Metal binding	59	1	Zinc By similarity
	Metal binding	62	1	Zinc By similarity
	Metal binding	151	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5W0D8-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: D423F185B35FBA9F	FASTA	335	37,451
	10 20 30 40 50 60 MLALGKLLEL TLTAHEPAEK TQVTPGGARL RWLGEGALEV RPAESEDCGL DLLLSAGIHG 70 80 90 100 110 120 NETAPIELLE RLLHGVANGK IKPRARVLFL FGNPAAIRKG ERFIEQDINR LFNGRHELSS 130 140 150 160 170 180 GFEALRAAEL EQFARVFFSK PGRNRLHYDL HTAIRGSKIE QFALYPYKEG RKHSRRELAR 190 200 210 220 230 240 LAAAGMEAVL LQSKSSITFS AFTYEQLEAE AFTLELGKAR PFGQNEQVNL DKLEERLIRI 250 260 270 280 290 300 IEATEPENES SLDGLQLFSV SREIIKHSDS FHLHLPADIE NFSELSKGYL LAEDLAEMRW 310 320 330 VVEEEGARII FPNPKVKNGL RAGILIVPDS GQRLG

References

[1]

"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000712 Genomic DNA. Translation: ABQ77598.1.
RefSeq	YP_001266782.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5194248.
GenomeReviews	Gene locus Pput_1440 in contig CP000712_GR.
KEGG	ppf:Pput_1440.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A5W0D8. KDEEFHM.
Family and domain databases
HAMAP	MF_00767. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_PSEP1

Accession

Primary (citable) accession number: A5W0D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)