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Protein

N-succinylarginine dihydrolase

Gene

astB

Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2.UniRule annotation

Catalytic activityi

N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2.UniRule annotation

Pathway:iL-arginine degradation via AST pathway

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Arginine N-succinyltransferase (Pput_1435), Arginine N-succinyltransferase (Pput_1436)
  2. N-succinylarginine dihydrolase (astB)
  3. no protein annotated in this organism
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101SubstrateUniRule annotation
Active sitei174 – 1741UniRule annotation
Binding sitei214 – 2141SubstrateUniRule annotation
Active sitei250 – 2501UniRule annotation
Binding sitei252 – 2521SubstrateUniRule annotation
Binding sitei365 – 3651SubstrateUniRule annotation
Active sitei371 – 3711NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism

Enzyme and pathway databases

BioCyciPPUT351746:GI26-1476-MONOMER.
UniPathwayiUPA00185; UER00280.

Names & Taxonomyi

Protein namesi
Recommended name:
N-succinylarginine dihydrolaseUniRule annotation (EC:3.5.3.23UniRule annotation)
Gene namesi
Name:astBUniRule annotation
Ordered Locus Names:Pput_1438
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006553 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449N-succinylarginine dihydrolasePRO_1000065733Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351746.Pput_1438.

Structurei

3D structure databases

ProteinModelPortaliA5W0D6.
SMRiA5W0D6. Positions 3-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 2810Substrate bindingUniRule annotation
Regioni137 – 1382Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the succinylarginine dihydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3724.
HOGENOMiHOG000226005.
KOiK01484.
OMAiFLNTQAK.
OrthoDBiEOG6B8XDW.

Family and domain databases

Gene3Di3.75.10.20. 1 hit.
HAMAPiMF_01172. AstB.
InterProiIPR007079. SuccinylArg_d-Hdrlase_AstB.
[Graphical view]
PfamiPF04996. AstB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03241. arg_catab_astB. 1 hit.

Sequencei

Sequence statusi: Complete.

A5W0D6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSYEVNFDG LVGPTHNYGG LSYGNVASQS NSQQASNPRE AARQGLAKMK
60 70 80 90 100
ALADMGFKQG VLAPQERPDV AALRRLGFSG SDADVIQRAA REAMPLLVAS
110 120 130 140 150
CSASSMWVAN AATVSPSADT ADGRVHFTAA NLNCKYHRSI EHPTTSRVLG
160 170 180 190 200
AMFNNEKHFA HHAALPAVAQ FGDEGAANHT RFCRAYGEAG VEFFVYGRSA
210 220 230 240 250
FDSRYPAPQK YPARQTLEAS QAVARLHGLS DDGVVYAQQN PAVIDQGVFH
260 270 280 290 300
NDVISVGNGE VLFYHEEAFL ETDAVLGQLR AKLASKGGNF QAICVPRAAV
310 320 330 340 350
AVEDAVRSYL FNSQLLSRDD GSMLLVVPEE CRNNERVWAY LGQLTSQGGP
360 370 380 390 400
VKEVKVFDLK QSMQNGGGPA CLRLRVALKE AELAAVNQGV IMTAPLYDTL
410 420 430 440
LQWVDRHYRD RLGEADLADP QLLVECRTAL DELTQILKLG SVYPFQRQP
Length:449
Mass (Da):48,817
Last modified:July 10, 2007 - v1
Checksum:iFE58561DB0608119
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ77596.1.
RefSeqiWP_012051580.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ77596; ABQ77596; Pput_1438.
KEGGippf:Pput_1438.
PATRICi19918939. VBIPsePut56420_1462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ77596.1.
RefSeqiWP_012051580.1. NC_009512.1.

3D structure databases

ProteinModelPortaliA5W0D6.
SMRiA5W0D6. Positions 3-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_1438.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ77596; ABQ77596; Pput_1438.
KEGGippf:Pput_1438.
PATRICi19918939. VBIPsePut56420_1462.

Phylogenomic databases

eggNOGiCOG3724.
HOGENOMiHOG000226005.
KOiK01484.
OMAiFLNTQAK.
OrthoDBiEOG6B8XDW.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00280.
BioCyciPPUT351746:GI26-1476-MONOMER.

Family and domain databases

Gene3Di3.75.10.20. 1 hit.
HAMAPiMF_01172. AstB.
InterProiIPR007079. SuccinylArg_d-Hdrlase_AstB.
[Graphical view]
PfamiPF04996. AstB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03241. arg_catab_astB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiASTB_PSEP1
AccessioniPrimary (citable) accession number: A5W0D6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: July 22, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.