A5W024 (ACEK_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Pput_1324

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000315270
Regions
	Nucleotide binding	318 – 324	7	ATP By similarity
Sites
	Active site	374	1	By similarity
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5W024 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 0A94EE44E65C1298	FASTA	571	65,954
	10 20 30 40 50 60 MSQPWPAVEI ARMILAGFDD YRDHFQRITL GARQRFEQAR WQDIQQAAAA RINLYEEKVA 70 80 90 100 110 120 EVNGWLRQAF AAEVLLDVEQ WPLVKNAYIH LIDPRLDDEL AETWYNSLFC SLFSHDLISD 130 140 150 160 170 180 GCMFIHTTRP SMRGRERAAQ TRTYRLDGSL RNLLRAVFAD YPFDMPYGDL EGDLARLEEQ 190 200 210 220 230 240 LRECLPDWVC KDPALAVELF VPVLYRNKGA YLVGRLYNSD EQWPLVIPLL HREGHGIEAD 250 260 270 280 290 300 ALITDEAEVS IIFSFTRSYF MADVPVPAEF VNFLKRILPG KHIAELYTSI GFYKQGKSEF 310 320 330 340 350 360 YRALINHLAS SDDRFVMAPG VRGMVMSVFT LPGFNTVFKI IKDRFSPSKT VDRATVIDKY 370 380 390 400 410 420 RLVKSVDRVG RMADTQEFAD FRFPRSKFEP DCLAELLEVA PSTVALEGDT VLIRHCWTER 430 440 450 460 470 480 RMTPLNLYLE QATEGQVLEA LEDYGLAIKQ LAAANIFPGD MLLKNFGVTR HGRVVFYDYD 490 500 510 520 530 540 EISFLTEVNF RHIPPPRYPE DEMSGEPWYS IGPHDVFPEE FPPFLFADMG QRRLFSRLHG 550 560 570 ELYDADYWKG LQAAIREGKV IDVFPYRRKA R

References

[1]

"Complete sequence of Pseudomonas putida F1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F1 / ATCC 700007.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000712 Genomic DNA. Translation: ABQ77484.1.
RefSeq	YP_001266668.1. NC_009512.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5W024.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5192661.
GenomeReviews	Gene locus Pput_1324 in contig CP000712_GR.
KEGG	ppf:Pput_1324.
PATRIC	19918703. VBIPsePut56420_1342.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	PPUT351746:PPUT_1324-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEP1

Accession

Primary (citable) accession number: A5W024

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program