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Protein

Ribosomal RNA large subunit methyltransferase F

Gene

rlmF

Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the adenine in position 1618 of 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPUT351746:GI26-1042-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase FUniRule annotation (EC:2.1.1.181UniRule annotation)
Alternative name(s):
23S rRNA mA1618 methyltransferaseUniRule annotation
rRNA adenine N-6-methyltransferaseUniRule annotation
Gene namesi
Name:rlmFUniRule annotation
Ordered Locus Names:Pput_1014
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006553 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Ribosomal RNA large subunit methyltransferase FPRO_0000349932Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_1014.

Structurei

3D structure databases

ProteinModelPortaliA5VZ65.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. METTL16/RlmF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EBA. Bacteria.
COG3129. LUCA.
HOGENOMiHOG000218605.
KOiK06970.
OMAiTEFHQGH.
OrthoDBiEOG6742SX.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01848. 23SrRNA_methyltr_F.
InterProiIPR016909. rRNA_lsu_MeTfrase_F.
IPR010286. rRNA_lsu_MeTfrase_F-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF029038. Mtase_YbiN_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

A5VZ65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPNKPTLHP RNRHQGRYDF PSLIKAHPDL ARFTITNPHG KPSIDFANPE
60 70 80 90 100
AVRVFNRALL KAQYGIQHWD IPADYLCPPI PGRADYIHVA ADLLADDNAG
110 120 130 140 150
DVPRGTQVRA LDVGVGANCI YPLLGHSDYR WRFLGSDIDP VALASAKAIV
160 170 180 190 200
QANGLGKAIT LRQQANRAHI LSGLLQEGER FDLTLCNPPF HASRDEATRG
210 220 230 240 250
SQRKWKNLGK QDPKRKLPVL NFGGQNNELW CEGGEIRFVT QLIGESVQYA
260 270 280 290 300
ERVLWFTSLV SKASNLPGIE AALKKSGAKA VRIIEMGQGQ KQSRMVAWSF
310
HDDAARQAWH AQRKSQA
Length:317
Mass (Da):35,201
Last modified:July 10, 2007 - v1
Checksum:iBDBE7D951937BEF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ77175.1.
RefSeqiWP_012051325.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ77175; ABQ77175; Pput_1014.
KEGGippf:Pput_1014.
PATRICi19918041. VBIPsePut56420_1019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ77175.1.
RefSeqiWP_012051325.1. NC_009512.1.

3D structure databases

ProteinModelPortaliA5VZ65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_1014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ77175; ABQ77175; Pput_1014.
KEGGippf:Pput_1014.
PATRICi19918041. VBIPsePut56420_1019.

Phylogenomic databases

eggNOGiENOG4105EBA. Bacteria.
COG3129. LUCA.
HOGENOMiHOG000218605.
KOiK06970.
OMAiTEFHQGH.
OrthoDBiEOG6742SX.

Enzyme and pathway databases

BioCyciPPUT351746:GI26-1042-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01848. 23SrRNA_methyltr_F.
InterProiIPR016909. rRNA_lsu_MeTfrase_F.
IPR010286. rRNA_lsu_MeTfrase_F-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF029038. Mtase_YbiN_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiRLMF_PSEP1
AccessioniPrimary (citable) accession number: A5VZ65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 10, 2007
Last modified: November 11, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.