A5VZ22 (GATA_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	Pput_0971

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120		PRO_1000015889
Sites
	Active site	76	1	Charge relay system By similarity
	Active site	151	1	Charge relay system By similarity
	Active site	175	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5VZ22 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: A715AD24E4FB7FF4	FASTA	483	51,531
	10 20 30 40 50 60 MHQLTLAEIA RGLADKSFSS EELTGALLAR IKQLDPQINS FISITDDLAL AQARAADARR 70 80 90 100 110 120 AAGETGVLLG APIAHKDLFC TNGVRTSCGS KMLDNFKAPY DATVVAKLAE AGMVTLGKTN 130 140 150 160 170 180 MDEFAMGSAN ESSHYGAVKN PWNLEHVPGG SSGGSAAAVA ARLLPATTGT DTGGSIRQPA 190 200 210 220 230 240 ALTNLTGLKP TYGRVSRWGM IAYASSLDQG GPLARTAEDC ALLLQGMAGF DAKDSTSIEE 250 260 270 280 290 300 PVPDYSASLN ASLQGLRIGL PKEYFGAGLD PRIADLVQAS VKELEKLGAV VKEISLPNMQ 310 320 330 340 350 360 HAIPAYYVIA PAEASSNLSR FDGVRFGYRC EEPKDLTDLY KRSRGEGFGV EVQRRIMVGT 370 380 390 400 410 420 YALSAGYYDA YYVKAQQIRR LIKNDFMAAF NDVDLILGPT TPNPAWKLGA KSSDPVAAYL 430 440 450 460 470 480 EDVYTITANL AGLPGLSMPA GFVDGLPVGV QLLAPYFQEG RLLNVAHRYQ QVTDWHTRAP NGF

References

[1]

"Complete sequence of Pseudomonas putida F1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F1 / ATCC 700007.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000712 Genomic DNA. Translation: ABQ77132.1.
RefSeq	YP_001266316.1. NC_009512.1.
3D structure databases
ProteinModelPortal	A5VZ22.
ModBase	Search...
Protein-protein interaction databases
STRING	351746.Pput_0971.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ77132; ABQ77132; Pput_0971.
GeneID	5192698.
KEGG	ppf:Pput_0971.
PATRIC	19917951. VBIPsePut56420_0974.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0154.
HOGENOM	HOG000116699.
KO	K02433.
OMA	RYDGVKY.
ProtClustDB	PRK00012.
Enzyme and pathway databases
BioCyc	PPUT351746:GI26-1067-MONOMER.
Family and domain databases
Gene3D	3.90.1300.10. 1 hit.
HAMAP	MF_00120. GatA.
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
PANTHER	PTHR11895. PTHR11895. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. gatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_PSEP1

Accession

Primary (citable) accession number: A5VZ22

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program