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Protein

Malate dehydrogenase

Gene

mdh

Organism
Pseudomonas putida (strain F1 / ATCC 700007)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331NADUniRule annotation
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei88 – 881SubstrateUniRule annotation
Binding sitei95 – 951NADUniRule annotation
Binding sitei120 – 1201SubstrateUniRule annotation
Binding sitei151 – 1511SubstrateUniRule annotation
Active sitei175 – 1751Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 136NADUniRule annotation
Nucleotide bindingi118 – 1203NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPPUT351746:GI26-712-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Pput_0687
OrganismiPseudomonas putida (strain F1 / ATCC 700007)
Taxonomic identifieri351746 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000006553 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Malate dehydrogenasePRO_1000191652Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351746.Pput_0687.

Structurei

3D structure databases

ProteinModelPortaliA5VY92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiNDYKDME.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

A5VY92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLTIVGAG LVGEAAAQII ARDELCRELV LMDVQGELAQ GKALDVWQAA
60 70 80 90 100
VDSGSDTHVH GGAKAEMLEG SELVVITAGV PRKPGQSRQD VLSTNLPILD
110 120 130 140 150
SIMADIKHHA PTATVLVVSN PVDVLTYRAW SVSGQGRDKV FGQAGVLDTA
160 170 180 190 200
RMKCFIAEQT GFSARDITAL VLGGHGDSMV PLMRYCQIGS VPLSHFLSSE
210 220 230 240 250
QIEQIVERTR KGGGEILGLK KTGSACDAPG VAIAQMVDAI GNGRNRILPA
260 270 280 290 300
VAILEGEYGR TDIAMGVPCV LAEKGLARVI ELPLDAQEQA MFDHSADQVA

RDIAEMKAL
Length:309
Mass (Da):32,656
Last modified:July 9, 2007 - v1
Checksum:iB512BB03B4EFEE94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ76852.1.
RefSeqiYP_001266036.1. NC_009512.1.

Genome annotation databases

EnsemblBacteriaiABQ76852; ABQ76852; Pput_0687.
KEGGippf:Pput_0687.
PATRICi19917365. VBIPsePut56420_0684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000712 Genomic DNA. Translation: ABQ76852.1.
RefSeqiYP_001266036.1. NC_009512.1.

3D structure databases

ProteinModelPortaliA5VY92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351746.Pput_0687.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ76852; ABQ76852; Pput_0687.
KEGGippf:Pput_0687.
PATRICi19917365. VBIPsePut56420_0684.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiNDYKDME.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciPPUT351746:GI26-712-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F1 / ATCC 700007.

Entry informationi

Entry nameiMDH_PSEP1
AccessioniPrimary (citable) accession number: A5VY92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2009
Last sequence update: July 9, 2007
Last modified: March 31, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.