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A5VXF1 (BIOB_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Pput_0388
OrganismPseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]
Taxonomic identifier351746 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352Biotin synthase HAMAP-Rule MF_01694
PRO_0000381560

Sites

Metal binding591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1031Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1341Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1941Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2661Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A5VXF1 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 9BF021E01536745A

FASTA35238,975
        10         20         30         40         50         60 
MSASTTATTR HDWSLAEVKA LFQQPFNDLL FQAQTVHRAH FNPNRVQVST LLSIKTGACP 

        70         80         90        100        110        120 
EDCKYCPQSG HYNTGLEKQK LMEVQKVLEE AARAKAIGST RFCMGAAWKH PSAKDMPYVL 

       130        140        150        160        170        180 
EMVKGVKAMG LETCMTLGKL DQEQTKALAH AGLDYYNHNL DTSPEFYGSI ITTRTYSERL 

       190        200        210        220        230        240 
QTLAYVRDAG MKICSGGILG MGESLDDRAG LLIQLANLPE HPESVPINML VKVAGTPLAE 

       250        260        270        280        290        300 
EEDVDPFDFI RMLAVARILM PKSHVRLSAG REQMNEQMQA LAFMAGANSI FYGEKLLTTA 

       310        320        330        340        350 
NPQADKDMQL FARLGIKPEA REEHADEVHQ AAIEQALVEQ RSSEMFYDAA TA 

« Hide

References

[1]"Complete sequence of Pseudomonas putida F1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F1 / ATCC 700007.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000712 Genomic DNA. Translation: ABQ76561.1.
RefSeqYP_001265745.1. NC_009512.1.

3D structure databases

ProteinModelPortalA5VXF1.
SMRA5VXF1. Positions 10-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351746.Pput_0388.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ76561; ABQ76561; Pput_0388.
GeneID5195060.
KEGGppf:Pput_0388.
PATRIC19916735. VBIPsePut56420_0390.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2299172.

Enzyme and pathway databases

BioCycPPUT351746:GI26-395-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PSEP1
AccessionPrimary (citable) accession number: A5VXF1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 10, 2007
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways