Reviewed, UniProtKB/Swiss-Prot A5VUF9 (BIOD_BRUO2)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	BOV_A0428

Organism

Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) [Complete proteome] [HAMAP]

Taxonomic identifier

444178 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Dethiobiotin synthetase HAMAP MF_00336		PRO_1000019549
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5VUF9-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: F7D5083F8F33E1DC	FASTA	212	22,988
	10 20 30 40 50 60 MNSRLIVTGT DTGIGKTVFS AALCHALGAA YWKPVQSGLE EETDSEIVAR LAQASPQRIL 70 80 90 100 110 120 PEAWRLNTPA SPHLSARLDG VEIRPEEMHI PATSLPLVIE GAGGLLVPLN DKTLFADLFA 130 140 150 160 170 180 IWRIPAILCA RAALGTINHT LLSLEAMRSR DIPVLGVAFI GEANEDTETT IAHLGRVKRL 190 200 210 GRLPLLDDLS PEKLHHSFAR NFHIDDFAGV AR

References

[1]	Paulsen I. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000709 Genomic DNA. Translation: ABQ62373.1.
RefSeq	YP_001257464.1.
3D structure databases
SMR	A5VUF9. Positions 4-189.
ModBase	Search...
Genome annotation databases
GeneID	5203077.
GenomeReviews	Gene locus BOV_A0428 in contig CP000709_GR.
KEGG	bov:BOV_A0428.
TIGR	BOV_A0428.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	WKPIQSG.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BRUO2

Accession

Primary (citable) accession number: A5VUF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)