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Protein

N-acetylneuraminate epimerase

Gene

nanM

Organism
Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in the beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses.UniRule annotation

Catalytic activityi

N-acetyl-alpha-neuraminate = N-acetyl-beta-neuraminate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei242 – 2421Proton acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciBOVI444178:GH2V-2449-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate epimeraseUniRule annotation (EC:5.1.3.24UniRule annotation)
Alternative name(s):
N-acetylneuraminate mutarotaseUniRule annotation
Short name:
Neu5Ac mutarotaseUniRule annotation
Sialic acid epimeraseUniRule annotation
Gene namesi
Name:nanMUniRule annotation
Ordered Locus Names:BOV_A0355
OrganismiBrucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Taxonomic identifieri444178 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000006383 Componenti: Chromosome II

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626UniRule annotationAdd
BLAST
Chaini27 – 388362N-acetylneuraminate epimerasePRO_0000333051Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati48 – 9245Kelch 1Add
BLAST
Repeati94 – 14754Kelch 2Add
BLAST
Repeati149 – 18638Kelch 3Add
BLAST
Repeati187 – 23246Kelch 4Add
BLAST
Repeati236 – 28550Kelch 5Add
BLAST
Repeati307 – 35650Kelch 6Add
BLAST
Repeati358 – 38730Kelch 7Add
BLAST

Sequence similaritiesi

Belongs to the NanM family.UniRule annotation
Contains 7 Kelch repeats.UniRule annotation

Keywords - Domaini

Kelch repeat, Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000218207.
KOiK17948.
OMAiGAAFTIQ.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
HAMAPiMF_01195. NanM. 1 hit.
InterProiIPR015915. Kelch-typ_b-propeller.
IPR019936. Mutatrotase_YjhT-like.
[Graphical view]
TIGRFAMsiTIGR03547. muta_rot_YjhT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5VU94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLIGAKRL AIGIAALAWS TGAVMASEHW PDLPVGIKNG AAARIGNMAY
60 70 80 90 100
VGLGSAGTDF YALDLNNPSK GWVKRANFIG PATNGAAMAA AGGKIFAFSG
110 120 130 140 150
NGKATPDAKS PIIFDTAYVY DPGSDGWSKL DTQTPVGLSG AKAVGLADGR
160 170 180 190 200
IAIFGGYNKE LFDKYLADVG AIDKDKEPEA YRKLVDSYMG MKPEAYRWND
210 220 230 240 250
EVLVYDPAGN NWGSLGANPF LPNCDPAMAT MGEGDFLLVS GEIKPGLRTP
260 270 280 290 300
EAKLVKIRDG AAHWQKVSDL PPLSGSEPQE GVAGAYAGKA GDDVLVAGGA
310 320 330 340 350
NFKGAQANAA AGKWFAHDGL AKSWRDDVYA FDGKDWKVAG KLPRGLAYGA
360 370 380
AFDAPGGLLV VGGEDRDGKA RKEVFLLKWD GKALSVEN
Length:388
Mass (Da):40,627
Last modified:July 10, 2007 - v1
Checksum:i6B1DBC00747DA663
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000709 Genomic DNA. Translation: ABQ62413.1.
RefSeqiWP_004688930.1. NC_009504.1.

Genome annotation databases

EnsemblBacteriaiABQ62413; ABQ62413; BOV_A0355.
KEGGibov:BOV_A0355.
PATRICi17857935. VBIBruOvi136990_0417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000709 Genomic DNA. Translation: ABQ62413.1.
RefSeqiWP_004688930.1. NC_009504.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ62413; ABQ62413; BOV_A0355.
KEGGibov:BOV_A0355.
PATRICi17857935. VBIBruOvi136990_0417.

Phylogenomic databases

HOGENOMiHOG000218207.
KOiK17948.
OMAiGAAFTIQ.

Enzyme and pathway databases

BioCyciBOVI444178:GH2V-2449-MONOMER.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
HAMAPiMF_01195. NanM. 1 hit.
InterProiIPR015915. Kelch-typ_b-propeller.
IPR019936. Mutatrotase_YjhT-like.
[Graphical view]
TIGRFAMsiTIGR03547. muta_rot_YjhT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNANM_BRUO2
AccessioniPrimary (citable) accession number: A5VU94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: September 7, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.