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A5VS60

- RUVC_BRUO2

UniProt

A5VS60 - RUVC_BRUO2

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi11 – 111MagnesiumUniRule annotation
    Metal bindingi71 – 711MagnesiumUniRule annotation
    Metal bindingi143 – 1431MagnesiumUniRule annotation
    Metal bindingi146 – 1461MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBOVI444178:GH2V-1645-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:BOV_1647
    OrganismiBrucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
    Taxonomic identifieri444178 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000006383: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 173173Crossover junction endodeoxyribonuclease RuvCPRO_1000002726Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi444178.BOV_1647.

    Structurei

    3D structure databases

    ProteinModelPortaliA5VS60.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012181.
    KOiK01159.
    OMAiRQVQEMV.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5VS60-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKETIRIIGI DPGLRRTGWG IVESLGNSLH FIGSGTVTSN AEMDLASRLC    50
    QLHEGLSKVL HEFMPHEAAV EHTFVNKDAT ATLKLGQARG IALLAPAQAG 100
    LPVAEYAPNA VKKAVIGVGH GEKQQIHMMV KVLMPRASFD TSDAADALAI 150
    AICHAHHRQS IVSARRMQAL LAG 173
    Length:173
    Mass (Da):18,455
    Last modified:July 10, 2007 - v1
    Checksum:iC1CF7EAF34FF2F42
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000708 Genomic DNA. Translation: ABQ61579.1.
    RefSeqiYP_001259557.1. NC_009505.1.

    Genome annotation databases

    EnsemblBacteriaiABQ61579; ABQ61579; BOV_1647.
    GeneIDi5201648.
    KEGGibov:BOV_1647.
    PATRICi17863344. VBIBruOvi136990_3055.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000708 Genomic DNA. Translation: ABQ61579.1 .
    RefSeqi YP_001259557.1. NC_009505.1.

    3D structure databases

    ProteinModelPortali A5VS60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 444178.BOV_1647.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ61579 ; ABQ61579 ; BOV_1647 .
    GeneIDi 5201648.
    KEGGi bov:BOV_1647.
    PATRICi 17863344. VBIBruOvi136990_3055.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012181.
    KOi K01159.
    OMAi RQVQEMV.
    OrthoDBi EOG6RG044.

    Enzyme and pathway databases

    BioCyci BOVI444178:GH2V-1645-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Paulsen I.
      Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25840 / 63/290 / NCTC 10512.

    Entry informationi

    Entry nameiRUVC_BRUO2
    AccessioniPrimary (citable) accession number: A5VS60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3