A5VS32 (A5VS32_BRUO2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116
Short name=dUTPase HAMAP MF_00116
EC=3.6.1.23 HAMAP MF_00116

Alternative name(s):
dUTP pyrophosphatase HAMAP MF_00116

Gene names

Name:	dut HAMAP MF_00116 EMBL ABQ61716.1
Ordered Locus Names:	BOV_1619

Organism

Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) [Complete proteome] [HAMAP]

Taxonomic identifier

444178 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116 SAAS SAAS008181
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116 SAAS SAAS008181
Cofactor	Magnesium By similarity. HAMAP MF_00116 SAAS SAAS008181
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family. HAMAP MF_00116

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_00116 SAAS SAAS008181
Ligand	Magnesium HAMAP MF_00116 SAAS SAAS008181 Metal-binding HAMAP MF_00116 SAAS SAAS008181
Molecular function	Hydrolase HAMAP MF_00116 SAAS SAAS008181 EMBL ABQ61716.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	93 – 95	3	Substrate binding By similarity HAMAP MF_00116
	Region	110 – 112	3	Substrate binding By similarity HAMAP MF_00116
Sites
	Binding site	106	1	Substrate By similarity HAMAP MF_00116
	Binding site	120	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_00116

Sequences

	Sequence		Length	Mass (Da)
	A5VS32 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 66588CEACC457E9C	FASTA	174	18,582
	10 20 30 40 50 60 MKHHLAGQLN FCRKGSLMTA ASSSAPTLGI IRLEHAKGLD LPAYETAGSA GMDLRAAVAE 70 80 90 100 110 120 DRQIVLLPGR RTLVPTGLIL EIPQGYEVQI RPRSGLAFKN GITCLNTPGT IDSDYRGEVK 130 140 150 160 170 VLLINLGDDD FRIERGMRIA QAVFAPVIQP KIEERAKISE TARGAGGFGS TGTA

References

[1]	Paulsen I. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000708 Genomic DNA. Translation: ABQ61716.1.
RefSeq	YP_001259529.1. NC_009505.1.
3D structure databases
ProteinModelPortal	A5VS32.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VS32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5202246.
GenomeReviews	Gene locus BOV_1619 in contig CP000708_GR.
KEGG	bov:BOV_1619.
PATRIC	17863286. VBIBruOvi136990_3026.
TIGR	BOV_1619.
Phylogenomic databases
HOGENOM	HBG436079.
OMA	LDLRACI.
ProtClustDB	PRK00601.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5VS32_BRUO2

Accession

Primary (citable) accession number: A5VS32

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)