eno

Functionⁱ

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityⁱ

2-phospho-D-glycerate = phosphoenolpyruvate + H₂O.UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotation

Enzyme regulationⁱ

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayⁱ: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

no protein annotated in this organism
Phosphoglycerate kinase (pgk)
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
Enolase (eno)
Pyruvate kinase (pyk)

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	154	SubstrateUniRule annotation	1
Binding siteⁱ	163	SubstrateUniRule annotation	1
Active siteⁱ	204	Proton donorUniRule annotation	1
Metal bindingⁱ	241	MagnesiumUniRule annotation	1
Metal bindingⁱ	284	MagnesiumUniRule annotation	1
Binding siteⁱ	284	SubstrateUniRule annotation	1
Metal bindingⁱ	311	MagnesiumUniRule annotation	1
Binding siteⁱ	311	SubstrateUniRule annotation	1
Active siteⁱ	336	Proton acceptorUniRule annotation	1
Binding siteⁱ	336	Substrate (covalent); in inhibited formUniRule annotation	1
Binding siteⁱ	387	SubstrateUniRule annotation	1

GO - Molecular functionⁱ

magnesium ion binding Source: InterPro
phosphopyruvate hydratase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glycolytic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Biological process	Glycolysis
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00187

UniPathwayⁱ

UPA00109; UER00187

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation) Alternative name(s): 2-phospho-D-glycerate hydro-lyaseUniRule annotation 2-phosphoglycerate dehydrataseUniRule annotation
Gene namesⁱ	Name:enoUniRule annotation Ordered Locus Names:BOV_1092
Organismⁱ	Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Taxonomic identifierⁱ	444178 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella ovis
Proteomesⁱ	UP000006383 Componentⁱ: Chromosome I

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00318
Secreted
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00318
Cell surface
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00318

Note:

Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componentⁱ

cell surface Source: UniProtKB-SubCell
extracellular region Source: UniProtKB-SubCell
phosphopyruvate hydratase complex Source: InterPro

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm, Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_1000019190	1 – 425	EnolaseAdd BLAST		425

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	A5VQQ7
SMRⁱ	A5VQQ7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	363 – 366	Substrate bindingUniRule annotation		4

Sequence similaritiesⁱ

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

HOGENOMⁱ	HOG000072173
KEGG Orthology (KO) More...KOⁱ	K01689
OMAⁱ	EFMIIPV

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313 enolase, 1 hit
Gene3Dⁱ	3.20.20.1201 hit 3.30.390.101 hit
HAMAPⁱ	MF_00318 Enolase, 1 hit
InterProⁱ	View protein in InterPro IPR000941 Enolase IPR036849 Enolase-like_C_sf IPR029017 Enolase-like_N IPR034390 Enolase-like_superfamily IPR020810 Enolase_C IPR020809 Enolase_CS IPR020811 Enolase_N
PANTHERⁱ	PTHR11902 PTHR11902, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113 Enolase_C, 1 hit PF03952 Enolase_N, 1 hit
PIRSFⁱ	PIRSF001400 Enolase, 1 hit
PRINTSⁱ	PR00148 ENOLASE
SFLDⁱ	SFLDG00178 enolase, 1 hit SFLDS00001 Enolase, 1 hit
SMARTⁱ	View protein in SMART SM01192 Enolase_C, 1 hit SM01193 Enolase_N, 1 hit
SUPFAMⁱ	SSF51604 SSF51604, 1 hit
TIGRFAMsⁱ	TIGR01060 eno, 1 hit
PROSITEⁱ	View protein in PROSITE PS00164 ENOLASE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

A5VQQ7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTAIIDIVGR EILDSRGNPT VEVDVVLEDG SFGRAAVPSG ASTGAHEAVE 
        60         70         80         90        100
LRDGGSRYLG KGVEKAVEVV NGKIFDAIAG MDAENQLLID QTLIDLDGSA 
       110        120        130        140        150
NKGNLGANAI LGVSLAVAKA AAQASGLPLY RYVGGTNAHV LPVPMMNIIN 
       160        170        180        190        200
GGAHADNPID FQEFMILPVG ATSIREAVRY GSEVFHTLKK RLKDAGHNTN 
       210        220        230        240        250
VGDEGGFAPN LKNAQAALDF IMESIEKAGF KPGEDIALGL DCAATEFFKD 
       260        270        280        290        300
GNYVYEGERK TRDPKAQAKY LAKLASDYPI VTIEDGMAED DWEGWKYLTD 
       310        320        330        340        350
LIGNKCQLVG DDLFVTNSAR LRDGIRLGVA NSILVKVNQI GSLSETLDAV 
       360        370        380        390        400
ETAHKAGYTA VMSHRSGETE DSTIADLAVA TNCGQIKTGS LARSDRTAKY 
       410        420 
NQLIRIEEEL GKQARYAGRS ALKLL

Length:425

Mass (Da):45,288

Last modified:July 10, 2007 - v1

Checksum:ⁱ18C5F7BD12A45C7E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000708 Genomic DNA Translation: ABQ61705.1
NCBI Reference Sequences More...RefSeqⁱ	WP_006012652.1, NC_009505.1

Genome annotation databases

EnsemblBacteriaⁱ	ABQ61705; ABQ61705; BOV_1092
KEGGⁱ	bov:BOV_1092

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A5VQQ7	N8LZ17	Brucella ovis IntaBari-2002-82-58	425	UniRef100_A5VQQ7	Cluster: Enolase	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A5VQQ7	A6X0L8 B2S5Y3 Q2YPV0 Q57D07 A9M5E5 Q8G0G3 A0A2K4NK65 C9VAT8 C0G6L8 C4IRI7 +51	Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168) Brucella abortus (strain S19) Brucella abortus (strain 2308) Brucella abortus biovar 1 (strain 9-941) Brucella canis (strain ATCC 23365 / NCTC 10854) Brucella suis biovar 1 (strain 1330) Brucella abortus Brucella neotomae 5K33 Brucella ceti str. Cudo Brucella abortus str. 2308 A And more	425	UniRef90_A6X0L8	Cluster: Enolase	62

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A5VQQ7	Q1D401 A0A1G7BPF2 A0A0H4WVC2 A0A250JYG7 F8CQP5 UPI00037C0E65 A0A1E3M0C5 A0A061NZ54 A0A061P629 A0A061N4W6 +2261	Myxococcus xanthus (strain DK 1622) Myxococcus virescens Myxococcus hansupus Myxococcus macrosporus DSM 14697 Myxococcus fulvus (strain ATCC BAA-855 / HW-1) Catelliglobosispora koreensis Sphingomonas sp. MCT13 Geomicrobium sp. JCM 19038 Geomicrobium sp. JCM 19039 Geomicrobium sp. JCM 19055 And more	432	UniRef50_Q1D401	Cluster: Enolase	2,272

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000708 Genomic DNA Translation: ABQ61705.1
NCBI Reference Sequences More...RefSeqⁱ	WP_006012652.1, NC_009505.1

3D structure databases

ProteinModelPortalⁱ	A5VQQ7
SMRⁱ	A5VQQ7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ABQ61705; ABQ61705; BOV_1092
KEGGⁱ	bov:BOV_1092

Phylogenomic databases

HOGENOMⁱ	HOG000072173
KEGG Orthology (KO) More...KOⁱ	K01689
OMAⁱ	EFMIIPV

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00187

UniPathwayⁱ

UPA00109; UER00187

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313 enolase, 1 hit
Gene3Dⁱ	3.20.20.1201 hit 3.30.390.101 hit
HAMAPⁱ	MF_00318 Enolase, 1 hit
InterProⁱ	View protein in InterPro IPR000941 Enolase IPR036849 Enolase-like_C_sf IPR029017 Enolase-like_N IPR034390 Enolase-like_superfamily IPR020810 Enolase_C IPR020809 Enolase_CS IPR020811 Enolase_N
PANTHERⁱ	PTHR11902 PTHR11902, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113 Enolase_C, 1 hit PF03952 Enolase_N, 1 hit
PIRSFⁱ	PIRSF001400 Enolase, 1 hit
PRINTSⁱ	PR00148 ENOLASE
SFLDⁱ	SFLDG00178 enolase, 1 hit SFLDS00001 Enolase, 1 hit
SMARTⁱ	View protein in SMART SM01192 Enolase_C, 1 hit SM01193 Enolase_N, 1 hit
SUPFAMⁱ	SSF51604 SSF51604, 1 hit
TIGRFAMsⁱ	TIGR01060 eno, 1 hit
PROSITEⁱ	View protein in PROSITE PS00164 ENOLASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ENO_BRUO2
Accessionⁱ	A5VQQ7Primary (citable) accession number: A5VQQ7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
	Last sequence update:	July 10, 2007
	Last modified:	February 28, 2018
	This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A5VQQ7 (ENO_BRUO2)

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Enolase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Enzyme regulationⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ