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A5VQP8

- LIPA_BRUO2

UniProt

A5VQP8 - LIPA_BRUO2

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Protein

Lipoyl synthase

Gene
lipA, BOV_1082
Organism
Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi86 – 861Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi93 – 931Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBOVI444178:GH2V-1081-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:BOV_1082
OrganismiBrucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Taxonomic identifieri444178 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000006383: Chromosome I

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Lipoyl synthaseUniRule annotationPRO_1000012196Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi444178.BOV_1082.

Structurei

3D structure databases

ProteinModelPortaliA5VQP8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A5VQP8-1 [UniParc]FASTAAdd to Basket

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MVTVLNTVNQ SGRLRHPEKA HRPDNEVLKK PDWIRVKAPV SRGYGETREI    50
VRSNKLVTVC EEAGCPNIRE CWEKKHATFM IMGEICTRAC AFCNISTGIP 100
NALDPNEPEN IAKAVKQMGL THVVITSVDR DDLADGGAHH FAEVIKAVRE 150
AAPATTIEIL TPDFLRKEGA LEIVVKARPD VFNHNLETVP SKYLKVRPGA 200
RYFHSIRLLQ RVKELDPTIF TKSGIMVGLG EERNEILQLM DDLRSADVDF 250
MTIGQYLQPT RKHHPVIRFV KPDEFKSFET IGKTKGFLLV ASSPLTRSSH 300
HAGEDFAKLK AAREALYASR AS 322
Length:322
Mass (Da):36,057
Last modified:July 10, 2007 - v1
Checksum:i7A0DFC4FE6281E99
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000708 Genomic DNA. Translation: ABQ61567.1.
RefSeqiYP_001259045.1. NC_009505.1.

Genome annotation databases

EnsemblBacteriaiABQ61567; ABQ61567; BOV_1082.
GeneIDi5201778.
KEGGibov:BOV_1082.
PATRICi17862084. VBIBruOvi136990_2446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000708 Genomic DNA. Translation: ABQ61567.1 .
RefSeqi YP_001259045.1. NC_009505.1.

3D structure databases

ProteinModelPortali A5VQP8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 444178.BOV_1082.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ61567 ; ABQ61567 ; BOV_1082 .
GeneIDi 5201778.
KEGGi bov:BOV_1082.
PATRICi 17862084. VBIBruOvi136990_2446.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci BOVI444178:GH2V-1081-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Paulsen I.
    Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25840 / 63/290 / NCTC 10512.

Entry informationi

Entry nameiLIPA_BRUO2
AccessioniPrimary (citable) accession number: A5VQP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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