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A5VQL8 (A5VQL8_BRUO2) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase RuleBase RU003777 HAMAP-Rule MF_00168
EC=2.4.2.29 RuleBase RU003777 HAMAP-Rule MF_00168
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168 EMBL ABQ61243.1
Ordered Locus Names:BOV_1052
OrganismBrucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) [Complete proteome] [HAMAP]
Taxonomic identifier444178 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella ›

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. RuleBase RU003777 SAAS SAAS004803 HAMAP-Rule MF_00168

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. SAAS SAAS004803 HAMAP-Rule MF_00168

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. RuleBase RU003777 SAAS SAAS004803 HAMAP-Rule MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. SAAS SAAS004803 HAMAP-Rule MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. RuleBase RU003777 HAMAP-Rule MF_00168

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site941Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3081Zinc By similarity HAMAP-Rule MF_00168
Binding site951Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
A5VQL8 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: C98BBF817FDA0B4D

FASTA37741,463
        10         20         30         40         50         60 
MTIENFGFKV LARDGAARQG KISMPRGVVR TPAFMPVGTA GTVKAMYMDQ VKELGADIIL 

        70         80         90        100        110        120 
GNTYHLMLRP GAERVARLGG LHEFGGWKGP ILTDSGGFQV MSLAQLRKLN EHGVTFRSHI 

       130        140        150        160        170        180 
DGKAYEMTPE RSIEIQGLLD SDIQMQLDEC VALPSPEKNT ERAMELSLRW AERCKVAFGD 

       190        200        210        220        230        240 
QPGKAMFGIV QGGDIARLRE RSAEALKAMD LKGYSVGGLA VGEPQEVMLD MLEVVCPILP 

       250        260        270        280        290        300 
TEKPRYLMGV GTPDDILKSV ARGIDMFDCV MPTRAGRHGL AFTRFGKVNL RNARHAEDHR 

       310        320        330        340        350        360 
PLDPQSDCPA SRDYSRAYLH HLVKSGEALG AMLLTWNNLA YYQYLMKGIR AAIADGNFSD 

       370 
FTAETTEGWA RGDMPAL

« Hide

References

[1]Paulsen I.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25840 / 63/290 / NCTC 10512.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000708 Genomic DNA. Translation: ABQ61243.1.
RefSeqYP_001259015.1. NC_009505.1.

3D structure databases

ProteinModelPortalA5VQL8.
SMRA5VQL8. Positions 5-365.
ModBaseSearch...

Protein-protein interaction databases

STRING444178.BOV_1052.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ61243; ABQ61243; BOV_1052.
GeneID5201798.
KEGGbov:BOV_1052.
PATRIC17862019. VBIBruOvi136990_2414.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycBOVI444178:GH2V-1078-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA5VQL8_BRUO2
AccessionPrimary (citable) accession number: A5VQL8
Entry history
Integrated into UniProtKB/TrEMBL: July 10, 2007
Last sequence update: July 10, 2007
Last modified: May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info