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Protein

Glutamate--tRNA ligase 1

Gene

gltX1

Organism
Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei253ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 1UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 1UniRule annotation
Short name:
GluRS 1UniRule annotation
Gene namesi
Name:gltX1UniRule annotation
Ordered Locus Names:BOV_0983
OrganismiBrucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Taxonomic identifieri444178 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000006383 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003676251 – 457Glutamate--tRNA ligase 1Add BLAST457

Proteomic databases

PRIDEiA5VQF9

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5VQF9
SMRiA5VQF9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi250 – 254"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252721
KOiK01885

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A5VQF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTVRFAPS PTGYIHIGNT RTALSNWLYA SKNNGKFILR YDDTDVERSK
60 70 80 90 100
DEYAQAIAVD LDWLGVRPDR VEYQSKRFDI YAKAVEKLKT AGLLYACYET
110 120 130 140 150
ADELERRRKL RLARRLPPVY GREALKLTDA EKAALEAEGR KPHWRFLLPN
160 170 180 190 200
FESDPFATQR TEVHWDDLVR GPQTVDLASM SDPILVREDG TYLYTLPSVV
210 220 230 240 250
DDIDMGVTHI IRGDDHVTNT GVQISIFKAL GATPPVFGHH NLLTTISGEG
260 270 280 290 300
LSKRTGALSV GSLREAGYEP MAVASLAILI GTSESVTAAP DMAALAEHFD
310 320 330 340 350
LASISKSSAK FDPSELDALN RSLLHEMPFE KAKPRLEALG ICGAKAESFW
360 370 380 390 400
LAVRGNLDRF SDVSHWWQVV SGDLPEAPDL SGEDRDFVRH AFDLLPPEPW
410 420 430 440 450
NGQTWKSWTE AVKSATGRKG KNLFMPLRLA LTGQAHGPEL ADLLVLVGLE

RTKSRRP
Length:457
Mass (Da):50,794
Last modified:March 24, 2009 - v2
Checksum:iF3D3CDDD74CCAC01
GO

Sequence cautioni

The sequence ABQ60984 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000708 Genomic DNA Translation: ABQ60984.1 Different initiation.
RefSeqiWP_002969100.1, NC_009505.1

Genome annotation databases

EnsemblBacteriaiABQ60984; ABQ60984; BOV_0983
KEGGibov:BOV_0983

Similar proteinsi

Entry informationi

Entry nameiSYE1_BRUO2
AccessioniPrimary (citable) accession number: A5VQF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: May 23, 2018
This is version 73 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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