A5VPM3 (AMPA_BRUO2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BOV_0680

Organism

Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) [Complete proteome] [HAMAP]

Taxonomic identifier

444178 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019888
Sites
	Active site	275	1	Potential
	Active site	349	1	Potential
	Metal binding	263	1	Manganese 2 By similarity
	Metal binding	268	1	Manganese 1 By similarity
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	286	1	Manganese 2 By similarity
	Metal binding	345	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5VPM3 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 4BAF152E29B92592	FASTA	497	52,604
	10 20 30 40 50 60 MSKRPSISFS EFEVPQKGVA VVLVAKGGGF ADEAAKAVGD AEKIARIADI SGFTGALGKT 70 80 90 100 110 120 AEAIETTPAG VEKIVLVGVG EPGKLGNDDW LKIGGAAFSQ IGNAERVTLT LALPETTIAG 130 140 150 160 170 180 DEAADVALGM VLRSYKFDRY KTRKSEENGE PKHAAKITIC VADTHSARKA FEVAEAVADG 190 200 210 220 230 240 VIQARNLVNE PANILGPVEF AEEAEKLEKL GVKVEVLGEK ELKKLGMGAL LGVAQGSVRP 250 260 270 280 290 300 PRLVVMEWHG AKGKEKPIAF VGKGVVFDTG GISIKPAANM EDMKGDMGGA AAVTGLMRAL 310 320 330 340 350 360 AGRKAKVNAI GVIGLVENMP DGNAQRPGDI VTSMSGQTIE VINTDAEGRL VLADALHYTN 370 380 390 400 410 420 DRFKPRFIIN LATLTGAVMV ALGQYHAGLF SNDDELADQL YDAGQSTGEK LWRLPLGTEY 430 440 450 460 470 480 DKMIDSKFAD MKNSAGRYGG SITAAQFLKR FVGETPWAHL DVAGTAMGSP ANEYNQSWAS 490 GFGVRLLDRL VRDQFES

References

[1]	Paulsen I. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25840 / 63/290 / NCTC 10512.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000708 Genomic DNA. Translation: ABQ60676.1.
RefSeq	YP_001258670.1. NC_009505.1.
3D structure databases
ProteinModelPortal	A5VPM3.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VPM3.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5201201.
GenomeReviews	Gene locus BOV_0680 in contig CP000708_GR.
KEGG	bov:BOV_0680.
PATRIC	17861186. VBIBruOvi136990_2010.
TIGR	BOV_0680.
Phylogenomic databases
HOGENOM	HBG742580.
OMA	NMHLMRY.
ProtClustDB	PRK00913.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BRUO2

Accession

Primary (citable) accession number: A5VPM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program