A5VPI9 (RSH_BRUO2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase rsh
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase

Gene names

Name:	rsh
Ordered Locus Names:	BOV_0645

Organism

Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) [Complete proteome] [HAMAP]

Taxonomic identifier

444178 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella ovis

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in adaptation of Brucella to its intracellular host environment By similarity.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Sequence similarities	Belongs to the RelA/SpoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	guanosine tetraphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 750	750	GTP pyrophosphokinase rsh		PRO_0000322564
Regions
	Domain	45 – 144	100	HD
	Domain	675 – 738	64	ACT

Sequences

	Sequence		Length	Mass (Da)
	A5VPI9 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: D717A5F11B802A6E	FASTA	750	83,914
	10 20 30 40 50 60 MMRQYELVER VQRYKPDVNE ALLNKAYVYA MQKHGSQKRA SGDPYFSHPL EVAAILTDMH 70 80 90 100 110 120 LDEATIAIAL LHDTIEDTTA TRQEIDQLFG PEIGKLVEGL TKLKKLDLVS KKAVQAENLR 130 140 150 160 170 180 KLLLAISEDV RVLLVKLADR LHNMRTLGVM REDKRLRIAE ETMDIYAPLA GRMGMQDMRE 190 200 210 220 230 240 ELEELAFRYI NPDAWRAVTD RLAELLEKNR GLLQKIETDL SEIFEKNGIK ASVKSRQKKP 250 260 270 280 290 300 WSVFRKMETK GLSFEQLSDI FGFRVMVDTV QDCYRALGLI HTTWSMVPGR FKDYISTPKQ 310 320 330 340 350 360 NDYRSIHTTI IGPSRQRIEL QIRTREMDEI AEFGVAAHSI YKDRGSANNP HKISTETNAY 370 380 390 400 410 420 AWLRQTIEQL SEGDNPEEFL EHTKLELFQD QVFCFTPKGR LIALPRGATP IDFAYAVHTD 430 440 450 460 470 480 IGDSCVGAKV NGRIMPLMTE LKNGDEVDII RSKAQVPPAA WESLVATGKA RAAIRRATRS 490 500 510 520 530 540 AVRKQYSGLG MRILERAFER AGKPFSKDIL KPGLPRLARK DVEDVLAAVG RGELPSTDVV 550 560 570 580 590 600 KAVYPDYQDT RVTTQNNPAK AGEKGWFNIQ NAAGMIFKVP EGGEGAAAKV DPAATTPKPG 610 620 630 640 650 660 KRALPIRGTN PDLPVRFAPE GAVPGDRIVG ILQPGAGITI YPIQSPALTA YDDQPERWID 670 680 690 700 710 720 VRWDIDDQMS ERFPARISVS AINSPGSLAE IAQIAAANDA NIHNLSMVRT APDFTEMIID 730 740 750 VEVWDLKHLN RIISQLKESA SVSSAKRVNG

References

[1]	Paulsen I. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25840 / 63/290 / NCTC 10512.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000708 Genomic DNA. Translation: ABQ60728.1.
RefSeq	YP_001258636.1. NC_009505.1.
3D structure databases
ProteinModelPortal	A5VPI9.
ModBase	Search...
Protein-protein interaction databases
STRING	444178.BOV_0645.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ60728; ABQ60728; BOV_0645.
GeneID	5201587.
KEGG	bov:BOV_0645.
PATRIC	17861118. VBIBruOvi136990_1976.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0317.
HOGENOM	HOG000018299.
KO	K01139.
OMA	ELDCIAT.
ProtClustDB	CLSK2754108.
Enzyme and pathway databases
BioCyc	BOVI444178:GH2V-683-MONOMER.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR026020. (p)ppGpp_Synthase. IPR012675. Beta-grasp_dom. IPR003607. HD/PDEase_dom. IPR004811. RelA/Spo_fam. IPR007685. RelA_SpoT. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSH_BRUO2

Accession

Primary (citable) accession number: A5VPI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program