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Protein

Modification methylase BabI

Gene

ccrM

Organism
Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBOVI444178:GH2V-493-MONOMER.

Protein family/group databases

REBASEi15978. M.BovORF495P.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BabI (EC:2.1.1.72)
Short name:
M.BabI
Alternative name(s):
Adenine-specific methyltransferase BabI
DNA methyltransferase CcrM
M.CcrMI ortholog
Gene namesi
Name:ccrM
Synonyms:babI
Ordered Locus Names:BOV_0495
OrganismiBrucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Taxonomic identifieri444178 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000006383: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Modification methylase BabIPRO_0000363192Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi444178.BOV_0495.

Structurei

3D structure databases

ProteinModelPortaliA5VP58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0863.
HOGENOMiHOG000125329.
KOiK13581.
OMAiFWHFEDG.
OrthoDBiEOG6FV84T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5VP58-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRSQVIEYPM SLVRLAHELP IEAPRTAWLD SIIKGDCVSA LERLPDHSVD
60 70 80 90 100
VIFADPPYNL QLGGDLHRPD QSMVSAVDDH WDQFESFQAY DAFTRAWLLA
110 120 130 140 150
CRRVLKPNGT IWVIGSYHNI FRVGTQLQDL GFWLLNDIVW RKTNPMPNFR
160 170 180 190 200
GRRFQNAHET LIWASRDQKG KGYTFNYEAM KAANDDVQMR SDWLFPICTG
210 220 230 240 250
SERLKDENGD KVHPTQKPEA LLARIMMASS KPGDVILDPF FGSGTTGAVA
260 270 280 290 300
KRLGRHFVGI EREQPYIDAA TARINAVEPL GKAELTVMTG KRAEPRVAFT
310 320 330 340 350
SVMEAGLLRP GTVLCDERRR FAAIVRADGT LTANGEAGSI HRIGARVQGF
360 370 380
DACNGWTFWH FEENGVLKPI DALRKIIREQ MAAAGA
Length:386
Mass (Da):43,292
Last modified:July 10, 2007 - v1
Checksum:i8662138DB587B6CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000708 Genomic DNA. Translation: ABQ61978.1.
RefSeqiYP_001258505.1. NC_009505.1.

Genome annotation databases

EnsemblBacteriaiABQ61978; ABQ61978; BOV_0495.
GeneIDi5201802.
KEGGibov:BOV_0495.
PATRICi17860791. VBIBruOvi136990_1813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000708 Genomic DNA. Translation: ABQ61978.1.
RefSeqiYP_001258505.1. NC_009505.1.

3D structure databases

ProteinModelPortaliA5VP58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi444178.BOV_0495.

Protein family/group databases

REBASEi15978. M.BovORF495P.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ61978; ABQ61978; BOV_0495.
GeneIDi5201802.
KEGGibov:BOV_0495.
PATRICi17860791. VBIBruOvi136990_1813.

Phylogenomic databases

eggNOGiCOG0863.
HOGENOMiHOG000125329.
KOiK13581.
OMAiFWHFEDG.
OrthoDBiEOG6FV84T.

Enzyme and pathway databases

BioCyciBOVI444178:GH2V-493-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25840 / 63/290 / NCTC 10512.

Entry informationi

Entry nameiMTB1_BRUO2
AccessioniPrimary (citable) accession number: A5VP58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: July 10, 2007
Last modified: February 4, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.