A5VP58 (MTB1_BRUO2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase BabI
Short name=M.BabI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase BabI
DNA methyltransferase CcrM
M.CcrMI ortholog

Gene names

Name:	ccrM
Synonyms:	babI
Ordered Locus Names:	BOV_0495

Organism

Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) [Complete proteome] [HAMAP]

Taxonomic identifier

444178 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Modification methylase BabI		PRO_0000363192

Sequences

	Sequence		Length	Mass (Da)
	A5VP58 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 8662138DB587B6CC	FASTA	386	43,292
	10 20 30 40 50 60 MRSQVIEYPM SLVRLAHELP IEAPRTAWLD SIIKGDCVSA LERLPDHSVD VIFADPPYNL 70 80 90 100 110 120 QLGGDLHRPD QSMVSAVDDH WDQFESFQAY DAFTRAWLLA CRRVLKPNGT IWVIGSYHNI 130 140 150 160 170 180 FRVGTQLQDL GFWLLNDIVW RKTNPMPNFR GRRFQNAHET LIWASRDQKG KGYTFNYEAM 190 200 210 220 230 240 KAANDDVQMR SDWLFPICTG SERLKDENGD KVHPTQKPEA LLARIMMASS KPGDVILDPF 250 260 270 280 290 300 FGSGTTGAVA KRLGRHFVGI EREQPYIDAA TARINAVEPL GKAELTVMTG KRAEPRVAFT 310 320 330 340 350 360 SVMEAGLLRP GTVLCDERRR FAAIVRADGT LTANGEAGSI HRIGARVQGF DACNGWTFWH 370 380 FEENGVLKPI DALRKIIREQ MAAAGA

References

[1]	Paulsen I. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25840 / 63/290 / NCTC 10512.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000708 Genomic DNA. Translation: ABQ61978.1.
RefSeq	YP_001258505.1. NC_009505.1.
3D structure databases
ProteinModelPortal	A5VP58.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VP58.
Protein family/group databases
REBASE	15978. M.BovORF495P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5201802.
GenomeReviews	Gene locus BOV_0495 in contig CP000708_GR.
KEGG	bov:BOV_0495.
PATRIC	17860791. VBIBruOvi136990_1813.
TIGR	BOV_0495.
Phylogenomic databases
HOGENOM	HBG501679.
OMA	ETMIWAS.
ProtClustDB	CLSK2518600.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
KO	K13581.
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BRUO2

Accession

Primary (citable) accession number: A5VP58

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program